BD Pharmingen- Purified Mouse Anti-Human IL-8_BD Pharmingen
产品信息
抗原名称
IL-8
宿主
Mouse IgG2b, κ
免疫原
Recombinant Human IL-8
简单描述
The G265-8 antibody reacts with both the 72 and 77 amino acid forms of human interleukin-8 (IL-8). The immunogen used to produce the G265-8 hybridoma was recombinant human IL-8.
商品描述
G265-5
The G265-8 antibody reacts with both the 72 and 77 amino acid forms of human interleukin-8 (IL-8). The immunogen used to produce the G265-8 hybridoma was recombinant human IL-8.
同种型
Mouse IgG2b, κ
克隆号
克隆 G265-5 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
ELISA Capture (Routinely Tested), Western blot (Reported)
反应种属
Human (QC Testing)
目标/特异性
IL-8
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(1)
1. Matsushima K, Oppenheim JJ. Interleukin 8 and MCAF: novel inflammatory cytokines inducible by IL 1 and TNF. Cytokine. 1989; 1(1):2-13. (Clone-specific).
参考图片
Human IL-8 sandwich ELISA standard curves. The curves were generated by sandwich ELISAs using Purified Mouse Anti-Human IL-8 (Cat. No. 554716) as capture antibody, doubling dilutions of recombinant IL-8 standards, and Biotin Mouse Anti-Human IL-8 (Cat. No. 554718) as detection antibody. Streptavidin-HRP (Cat. No. 554066) plus TMB substrate (Cat. No. 555214) were used to develop the ELISA. Note that several distinct N-termini exist in IL-8; standard curves were generated with recombinant 72 amino acid (open square curve) or 77 amino acid (filled circle curve) IL-8 standards.
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