产品信息
荧光素标记
PE
抗原名称
CD25 (IL-2 Receptor a)
宿主
Mouse BALB/c IgG1, κ
免疫原
Rat T blasts from mixed-lymphocyte reactions
简单描述
The OX-39 monoclonal antibody specifically binds to the α chain of the IL-2 Receptor (IL-2Rα, IL2RA) on activated T cells, T regulatory cells, and thymic and splenic dendritic cells. CD25 has also been detected on rat intestinal epithelial cells. It has been reported that OX-39 mAb weakly blocks binding of IL-2 to T-cell blasts and that it blocks IL-2 stimulated epithelial cell migration in an in vitro model of wound healing.
商品描述
OX-39
The OX-39 monoclonal antibody specifically binds to the α chain of the IL-2 Receptor (IL-2Rα, IL2RA) on activated T cells, T regulatory cells, and thymic and splenic dendritic cells. CD25 has also been detected on rat intestinal epithelial cells. It has been reported that OX-39 mAb weakly blocks binding of IL-2 to T-cell blasts and that it blocks IL-2 stimulated epithelial cell migration in an in vitro model of wound healing.
同种型
Mouse BALB/c IgG1, κ
克隆号
克隆 OX-39 (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Rat (QC Testing)
目标/特异性
CD25 (IL-2 Receptor α)
背景
别名
Interleukin-2 receptor alpha chain; IL-2RA; IL-2Rα; Il2ra; IL-2R p55
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(5)
1. Dignass AU, Podolsky DK. Interleukin 2 modulates intestinal epithelial cell function in vitro. Exp Cell Res. 1996; 225(2):422-429. (Clone-specific: Blocking).
2. Hillebrands JL, Whalen B, Visser JT, et al. A regulatory CD4+ T cell subset in the BB rat model of autoimmune diabetes expresses neither CD25 nor Foxp3.. J Immunol. 2006; 177(11):7820-32. (Biology).
3. Josien R, Heslan M, Soulillou JP, Cuturi MC. Rat spleen dendritic cells express natural killer cell receptor protein 1 (NKR-P1) and have cytotoxic activity to select targets via a Ca2+-dependent mechanism. J Exp Med. 1997; 186(3):467-472. (Clone-specific).
4. Paterson DJ, Jefferies WA, Green JR. Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts. Mol Immunol. 1987; 24(12):1281-1290. (Immunogen: Blocking, Immunohistochemistry, Immunoprecipitation).
5. Tellides G, Dallman MJ, Kupiec-Weglinski JW, Diamantstein T, Morris PJ. Functional blocking of the interleukin-2 receptor (IL-2R) may be important in the efficacy of IL-2R antibody therapy. Transplant Proc. 1987; 19(5):4231-4233. (Clone-specific: Blocking).
参考图片
本文来自投稿，不代表本人立场，如若转载，请注明出处：http://www.iamyjet.com/kangti/9736.html