BD Pharmingen- Purified Mouse Anti-Human VEGF_BD Pharmingen
产品信息
抗原名称
VEGF
宿主
Mouse IgG2b, κ
简单描述
Vascular endothelial growth factor (VEGF) is a heparin-binding, dimeric protein related to the PDGF/sis family of growth factors. Major sources of VEGF include pituitary cells, monocytes/macrophages, smooth muscle, and keratinocytes. VEGF is a mitogen for endothelial cells, activates and is chemoattractant for monocytes, enhances blood vessel permeability, and is a pro-coagulant. Human VEGF occurs in several molecular variants arising by alternative splicing of the mRNA. The splice forms of VEGF differ in biological properties. VEGF is a homodimeric heavily glycosylated protein of 46-48 kDa (18-25 kDa subunits). Glycosylation is not required, however, for biological activity. The subunits are linked by disulphide bonds. Different isoforms of VEGF have different properties in vitro and this may apply also to their in vivo functions.
商品描述
G153-694
Vascular endothelial growth factor (VEGF) is a heparin-binding, dimeric protein related to the PDGF/sis family of growth factors. Major sources of VEGF include pituitary cells, monocytes/macrophages, smooth muscle, and keratinocytes. VEGF is a mitogen for endothelial cells, activates and is chemoattractant for monocytes, enhances blood vessel permeability, and is a pro-coagulant. Human VEGF occurs in several molecular variants arising by alternative splicing of the mRNA. The splice forms of VEGF differ in biological properties. VEGF is a homodimeric heavily glycosylated protein of 46-48 kDa (18-25 kDa subunits). Glycosylation is not required, however, for biological activity. The subunits are linked by disulphide bonds. Different isoforms of VEGF have different properties in vitro and this may apply also to their in vivo functions.
同种型
Mouse IgG2b, κ
克隆号
克隆 G153-694 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Immunohistochemistry-formalin (antigen retrieval required) (Routinely Tested), Immunofluorescence (Tested During Development), Immunoprecipitation/Western blot (Reported)
反应种属
Human (QC Testing)
目标/特异性
VEGF
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(6)
1. Andersson J, Abrams J, Bjork L, et al. Concomitant in vivo production of 19 different cytokines in human tonsils. Immunology. 1994; 83(1):16-24. (Biology).
2. Andersson U, Andersson J. Immunolabeling of cytokine-producing cells in tissues and in suspension. In: Fradelizie D, Emelie D, ed. Cytokine Producing Cells. Paris: Inserm; 1994:32-49.
3. Fernandez V, Andersson J, Andersson U, Troye-Blomberg M. Cytokine synthesis analyzed at the single-cell level before and after revaccination with tetanus toxoid. Eur J Immunol. 1994; 24(8):1808-1815. (Clone-specific: Immunohistochemistry).
4. Litton M, Andersson J, Bjork L, Fehniger T, Ulfgren AK, Andersson U. Cytoplasmic cytokine staining in individual cells. In: Debets and Savelkoul, ed. Human Cytokine Protocols. Humana Press; 1996.
5. Norrby-Teglund A, Norgren M, Holm SE, Andersson U, Andersson J. Similar cytokine induction profiles of a novel streptococcal exotoxin, MF, and pyrogenic exotoxins A and B. Infect Immun. 1994; 62(9):3731-3738. (Clone-specific: Immunohistochemistry).
6. Skansén-Saphir U, Andersson J, Björk L, Andersson U. Lymphokine production induced by streptococcal pyrogenic exotoxin-A is selectively down-regulated by pooled human IgG. Eur J Immunol. 1994; 24(4):916-922. (Clone-specific: Immunohistochemistry).
参考图片
VEGF Immonohistochemical staining on human colon tissue. Formalin fixed paraffin embedded human colon tissue was pretreated with BD Retrievagen A (Cat. No. 550524) and then stained with either Purified Mouse IgG2b, κ Isotype Control (Cat. No. 557351; Left panel) or Purified Mouse Anti-Human VEGF (Cat. No. 555036; Right Panel).
VEGF Immonohistochemical staining on human colon tissue. Formalin fixed paraffin embedded human colon tissue was pretreated with BD Retrievagen A (Cat. No. 550524) and then stained with either Purified Mouse IgG2b, κ Isotype Control (Cat. No. 557351; Left panel) or Purified Mouse Anti-Human VEGF (Cat. No. 555036; Right Panel).
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