BD Pharmingen- Purified Mouse Anti-Human MIG_BD Pharmingen
产品信息
抗原名称
CXCL9 (MIG)
宿主
Mouse IgG1, κ
免疫原
Recombinant Human MIG Protein
简单描述
The B8-11 monoclonal antibody specifically recognizes monokine induced by gamma interferon (MIG), which is also known as chemokine (C-X-C motif) ligand 9 (CXCL9). MIG is a chemotactic cytokine that belongs to the CXC subfamily of chemokines. MIG is predominantly expressed by monocytes, macrophages, hepatocytes, and endothelial cells in response to interferon-gamma (IFN-γ) stimulation. It is primarily involved in the recruitment of activated T cells. MIG/CXCL9, and the closely-related IP10/CXCL10 and I-TAC/CXCL11 chemokines, exert their biological activities by binding to and signaling through the CXCR3 (CD183) chemokine receptor. CXCR3 is expressed on multiple cell types but predominantly on memory and effector T cells. The CXCL9 and CXCR3 interaction plays an important role in the onset of inflammation and is implicated in the pathogenesis of T-cell mediated immunity in several disease models.
商品描述
B8-11
The B8-11 monoclonal antibody specifically recognizes monokine induced by gamma interferon (MIG), which is also known as chemokine (C-X-C motif) ligand 9 (CXCL9). MIG is a chemotactic cytokine that belongs to the CXC subfamily of chemokines. MIG is predominantly expressed by monocytes, macrophages, hepatocytes, and endothelial cells in response to interferon-gamma (IFN-γ) stimulation. It is primarily involved in the recruitment of activated T cells. MIG/CXCL9, and the closely-related IP10/CXCL10 and I-TAC/CXCL11 chemokines, exert their biological activities by binding to and signaling through the CXCR3 (CD183) chemokine receptor. CXCR3 is expressed on multiple cell types but predominantly on memory and effector T cells. The CXCL9 and CXCR3 interaction plays an important role in the onset of inflammation and is implicated in the pathogenesis of T-cell mediated immunity in several disease models.
同种型
Mouse IgG1, κ
克隆号
克隆 B8-11 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
ELISA Capture (Routinely Tested), Intracellular block/flow cytometry, Intracellular staining (flow cytometry) (Tested During Development)
反应种属
Human (QC Testing)
目标/特异性
CXCL9 (MIG)
背景
别名
C-X-C motif chemokine 9; CMK; crg-10; Humig; SCYB9
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(4)
1. Farber JM. A macrophage mRNA selectively induced by gamma-interferon encodes a member of the platelet factor 4 family of cytokines. Proc Natl Acad Sci U S A. 1990; 87(14):5238-5242. (Biology).
2. Farber JM. HuMig: a new human member of the chemokine family of cytokines. Biochem Biophys Res Commun. 1993; 192(1):223-230. (Biology).
3. Liao F, Rabin RL, Yannelli JR, Koniaris LG, Vanguri P, Farber JM. Human Mig chemokine: biochemical and functional characterization. J Exp Med. 1995; 182(5):1301-1314. (Biology).
4. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Clone-specific: Flow cytometry, IC/FCM Block, Immunofluorescence).
参考图片
Human MIG Standard Curve
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