Interleukin-2 (IL-2) is a potent, multifunctional cytokine produced by activated T lymphocytes. It is responsible for the activities attributed to T cell growth factor, thymocyte stimulation factor, thymocyte mitogenesis factor, killer helper factor, and T cell replacing factor. IL-2 stimulates the growth and differentiation of T cells, as well as cytokine production by T cells. In addition, IL-2 can contribute to the activation of monocytes/macrophages and NK and LAK cells and can augment B cell growth and differentiation. Formulation and Purity Recombinant rat IL-2 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution (pH 7.2) and containing 2 mg/ml  biotechnology grade, low-endotoxin bovine serum albumin, glycerol and with no preservatives. The endotoxin level is ≤ 0.1 ng per μg of rat IL-2 protein, as measured in a LAL chromogenic assay. This recombinant rat IL-2 is a single band of approximately 16.5 kD as determined by SDS-PAGE analysis on 10 - 20% gels run under reducing conditions.

(RUO)

100 µg/ml

ELISA Standard (Routinely Tested), Bioassay (Tested During Development)

Rat (QC Testing)

IL-2

Frozen aqueous buffered solution containing BSA and glycerol.

Development References(7) 1. Gillis S, Ferm MM, Ou W, Smith KA. T cell growth factor: parameters of production and a quantitative microassay for activity. J Immunol. 1978; 120(6):2027-2032. (Methodology). 2. Kashima N, Nishi-Takaoka C, Fujita T, et al. Unique structure of murine interleukin-2 as deduced from cloned cDNAs. Nature. 1985; 313(6001):402-404. (Biology). 3. McKnight AJ, Classon BJ. Biochemical and immunological properties of rat recombinant interleukin-2 and interleukin-4. Immunology. 1992; 75(2):286-292. (Biology). 4. Smith, KA. Interleukin-2: inception, impact, and implications. Science. 1988; 240(4856):1169-1176. (Biology). 5. Thompson LP, Di Sabato G. Purification and characterization of two forms of rat interleukin-2. Cell Immunol. 1988; 114(1):12-26. (Biology). 6. Watson J, Mochizuki D. Interleukin 2: a class of T cell growth factors. Immunol Rev. 1980; 51:257-278. (Biology). 7. Yokota T, Arai N, Lee F, Rennick D, Mosmann T, Arai K. Use of a cDNA expression vector for isolation of mouse interleukin 2 cDNA clones: expression of T-cell growth-factor activity after transfection of monkey cells. Proc Natl Acad Sci U S A. 1985; 82(1):68-72. (Biology).