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BD OptEIA- Mouse IL-5 ELISA Set_BD Pharmingen

产品信息
抗原名称
IL-5
克隆号
(RUO)
BD化合物表
  • 描述
    数量/尺寸
    零件号
    EntrezGene ID
  • Recombinant Mouse IL-5 Lyophilized Standard
    N/A
    51-26526E
    N/A
  • Detection Antibody Biotin Anti-Mouse IL-5
    N/A
    51-26522E
    N/A
  • Capture Antibody Purified Anti-Mouse IL-5
    N/A
    51-26521E
    N/A
  • Enzyme Reagent Streptavidin-horseradish peroxidase conjugate (SAv-HRP)
    N/A
    51-9002813
    N/A
应用
实验应用
ELISA (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
IL-5
文献
文献
研发参考(4) 1. Abrams J. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001; 1:6.20-6.21. (Biology). 2. Fujihashi K, McGhee JR, Beagley KW, et al. Cytokine-specific ELISPOT assay. Single cell analysis of IL-2, IL-4 and IL-6 producing cells. J Immunol Methods. 1993; 160(2):181-189. (Biology). 3. Litton MJ, Sander B, Murphy E, O'Garra A, Abrams JS. Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B. J Immunol Methods. 1994; 175(1):47-58. (Biology). 4. Sander B, Hoiden I, Andersson U, Moller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993; 166(2):201-214. (Biology).

参考图片

This standard curve is fordemonstration only. A standard curve must be run with each assay. \"Typical Standard Curve\" and 20-plate yield were obtained in the BD Biosciences Pharmingen laboratory, using the recommended procedure and manual plate washing.

Serial dilutions within the plate may also be performed by pipetting 100 µL of Assay Diluent into each standard well except the highest (1000 pg/mL), then adding 100 µL of the 1000 pg/mL standard to both that well and the 500 pg/mL well, mixing the well contents by rinsing the pipette tip, and adding 100 µL of the 500 pg/mL standard to the 250 pg/mL well. Continue these dilutions to the 15.6 pg/mL standard well, out of which the extra 100 µL should be discarded.

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