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BD OptEIA- Mouse MCP-1 ELISA Set_BD Pharmingen

产品信息
抗原名称
MCP-1
克隆号
(RUO)
BD化合物表
  • 描述
    数量/尺寸
    零件号
    EntrezGene ID
  • Detection Antibody HRP Anti-Mouse MCP-1
    N/A
    51-26657E
    44949
  • Recombinant Mouse MCP-1 Lyophilized Standard
    N/A
    51-26656E
    44949
  • Capture Antibody Purified Anti-Mouse MCP-1
    N/A
    51-26651E
    44949
应用
实验应用
ELISA (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
MCP-1
文献
文献
研发参考(12) 1. Boring L, Gosling J, Monteclaro FS, Lusis AJ, Tsou CL, Charo IF. Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors: evidence for two closely linked C-C chemokine receptors on chromosome 9. J Biol Chem. 1996; 271(13):7551-7558. (Biology). 2. Cochran BH, Reffel AC, Stiles CD. Molecular cloning of gene sequences regulated by platelet-derived growth factor. Cell. 1983; 33(3):939-947. (Biology). 3. Ernst CA, Zhang YJ, Hancock PR, Rutledge BJ, Corless CL, Rollins BJ. Biochemical and biologic characterization of murine monocyte chemoattractant protein-1. Identification of two functional domains.. J Immunol. 1994; 152(7):3541-3549. (Biology). 4. Luo Y, Laning J, Hayashi M, Hancock PR, Rollins B, Dorf ME. Serologic analysis of the mouse beta chemokine JE/monocyte chemoattractant protein-1. J Immunol. 1994; 153(8):3708-3716. (Biology). 5. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Biology). 6. Rollins BJ, Morrison ED, Stiles CD. Cloning and expression of JE, a gene inducible by platelet-derived growth factor and whose product has cytokine-like properties. Proc Natl Acad Sci U S A. 1988; 85(11):3738-3742. (Biology). 7. Rollins BJ. Chemokines. Blood. 1997; 90(3):909-928. (Biology). 8. Sakanashi Y, Takeya M, Yoshimura T, Feng L, Morioka T, Takahashi K. Kinetics of macrophage subpopulations and expression of monocyte chemoattractant protein-1 (MCP-1) in bleomycin-induced lung injury of rats studied by a novel monoclonal antibody against rat MCP-1. J Leukoc Biol. 1994; 56(6):741-750. (Biology). 9. Vaddi K, Keller M, Newton RC. The chemokine factsbook. San Diego: Academic Press; 1997:205 p. 10. Wang H, Nemoto-Sasaki Y, Kondo T, Akiyama M, Mukaida N. Potential involvement of monocyte chemoattractant protein (MCP)-1/CCL2 in IL-4-mediated tumor immunity through inducing dendritic cell migration into the draining lymph nodes. Int J Immunopharmacol. 2003; 3(5):627-642. (Biology). 11. Yoshimura T, Takeya M, Takahashi K. Molecular cloning of rat monocyte chemoattractant protein-1 (MCP-1) and its expression in rat spleen cells and tumor cell lines. Biochem Biophys Res Commun. 1991; 174(2):504-509. (Biology). 12. Yoshimura T, Yuhki N, Moore SK, Appella E, Lerman MI, Leonard EJ. Human monocyte chemoattractant protein-1 (MCP-1). Full-length cDNA cloning, expression in mitogen-stimulated blood mononuclear leukocytes, and sequence similarity to mouse competence gene JE. FEBS Lett. 1989; 244(2):487-493. (Biology).

参考图片

This standard curve is fordemonstration only. A standard curve must be run with each assay. \"Typical Standard Curve\" and 20-plate yield were obtained in the BD Biosciences Pharmingen laboratory, using the recommended procedure and manual plate washing.

Serial dilutions within the plate may also be performed by pipetting 100 µL of Assay Diluent into each standard well except the highest (1000 pg/mL), then adding 100 µL of the 1000 pg/mL standard to both that well and the 500 pg/mL well, mixing the well contents by rinsing the pipette tip, and adding 100 µL of the 500 pg/mL standard to the 250 pg/mL well. Continue these dilutions to the 15.6 pg/mL standard well, out of which the extra 100 µL should be discarded.

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