BD Pharmingen- Purified Mouse Anti-Human CD29_BD Pharmingen
产品信息
抗原名称
CD29 (Integrin β1)
宿主
Mouse BALB/c IgG1, κ
免疫原
Purified Human α5β1 Fibronectin Receptor
简单描述
The MAR4 monoclonal antibody specifically binds to CD29. CD29 is a 130 kDa integrin β1 subunit that is expressed as a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. The β1 subunit has a broad tissue distribution; it is expressed on lymphocytes, monocytes and weakly on granulocytes, but not on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.
商品描述
MAR4
The MAR4 monoclonal antibody specifically binds to CD29. CD29 is a 130 kDa integrin β1 subunit that is expressed as a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. The β1 subunit has a broad tissue distribution; it is expressed on lymphocytes, monocytes and weakly on granulocytes, but not on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.
同种型
Mouse BALB/c IgG1, κ
克隆号
克隆 MAR4 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Human (QC Testing), Rhesus,Cynomolgus,Baboon (Tested in Development)
目标/特异性
CD29 (Integrin β1)
背景
别名
ITGB1; Integrin beta-1; Integrin β1; FNRB; GPIIA; ITB1; MDF2; MSK12; VLA-4β
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(5)
1. Hemler ME. VLA proteins in the integrin family: structures, functions, and their role on leukocytes. Annu Rev Immunol. 1990; 8:365-400. (Biology).
2. Hynes RO. Integrins: versatility, modulation, and signaling in cell adhesion. Cell. 1992; 69(1):11-25. (Biology).
3. Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
4. Pellegrini R, Bazzini P, Tosi E, et al. Production and characterization of two monoclonal antibodies directed against the integrin beta 1 chain. Tumori. 1992; 78(1):1-4. (Immunogen).
5. Tanaka Y, Aso M, Takada Y. CD29 Workshop Panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:357-360.
参考图片
Flow cytometric analysis of CD29 expression on human peripheral blood lymphocytes. Whole blood was stained with Purified Mouse Anti-Human CD29 antibody (Cat. No. 555442; solid line histogram) or with a Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746; dashed line histogram). Secondary staining was carried out with FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988) and erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.
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