BD Pharmingen- PE Mouse Anti-Human CD36_BD Pharmingen
产品信息
荧光素标记
PE
抗原名称
CD36
宿主
Mouse IgM, κ
免疫原
Human Platelet Extracts
简单描述
The CB38 monoclonal antibody specifically binds to CD36. CD36 is a 88 kDa glycoprotein IV (GPIV), the receptor for extracellular matrix proteins such as collagen and thrombospondin. CD36 is known to mediate the adhesion of
Plasmodium falciparum
. CD36 antigen is expressed on
monocytes, platelets, endothelial cells, and some human tumor cell lines but not on lymphocytes and granulocytes. It is a very early marker of erythroid differentiation. CD36 antibody induces degranulation, release of ATP and serotonin, increase in [Ca2+]ι, and tyrosine phosphorylation of a substrate protein of 130 kDa.
商品描述
CB38
The CB38 monoclonal antibody specifically binds to CD36. CD36 is a 88 kDa glycoprotein IV (GPIV), the receptor for extracellular matrix proteins such as collagen and thrombospondin. CD36 is known to mediate the adhesion of
Plasmodium falciparum
. CD36 antigen is expressed on
monocytes, platelets, endothelial cells, and some human tumor cell lines but not on lymphocytes and granulocytes. It is a very early marker of erythroid differentiation. CD36 antibody induces degranulation, release of ATP and serotonin, increase in [Ca2+]ι, and tyrosine phosphorylation of a substrate protein of 130 kDa.
同种型
Mouse IgM, κ
克隆号
克隆 CB38 (also known as NL07) (RUO)
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
20 µl
反应种属
Human (QC Testing)
目标/特异性
CD36
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(3)
1. Alessio M, Greco NJ, Primo L, et al. Platelet activation and inhibition of malarial cytoadherence by the anti-CD36 IgM monoclonal antibody NL07. Blood. 1993; 82(12):3637-3647. (Biology).
2. Alessio M, Roggero S, Bussolino F, Saitta M, Malavasi F. Characterization of the murine monoclonal antibody NL07 specific for the human thrombospondin receptor (CD36 molecule). Curr Stud Hematol Blood Transfus. 1991; 58:182-186. (Biology).
3. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
参考图片
Profile of peripheral blood platelets analyzed on a FACScan (BDIS, San Jose, CA)
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