产品信息
商品描述
Coelenterazine is a luminescent enzyme substrate, used for monitoring reporter genes in BRET, ELISA and HTS techniques.
分子量
423.46
纯度
98%
可溶性
Ethanol : 2 mg/mL (4.72 mM; Need ultrasonic; DMSO can inactivate Coelenterazine‘s activity)
外观
yellow solid
In vitro(体外研究)
When monitoring various biological processes, coelenterazine (CTZ) can be catalyzed by luciferases, such as the sea pansy Renilla reniformis (Rluc) and the marine copepod Gaussia princeps (Gluc), which results in emission of blue light. In Gli36-Gluc- or Gli36-Rluc-expressing Gli36 cells, coelenterazine (8 μM/ml) shows a bioluminescence signal. In RIN-5F, 4T1, HCT 116, HEK 293, 293FT, HeLa and INS-1 cells, coelenterazine can be used to assay superoxide anion concentrations.
In vivo(体内研究)
In 4T1-implanted mouse, Coelenterazine detects cancer-associated superoxide anion and used to evaluate the role of superoxide anion in cancer cell biology and its role in pathology.
背景
别名
腔肠素
分子式
C26H21N3O3
CAS号
55779-48-1
制备和贮存
保存方式
Store at -20℃ for one year（Powder）；Store at 2-4℃ for two weeks；Store at -20℃ for six months after dissolution.
参考图片
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