Gene Bridges - 重组公司
使用Red / ET重组工程允许在任何选定位置克隆，亚克隆和修饰DNA。它允许设计任何大小的DNA分子，包括非常大的DNA分子，如BAC或大肠杆菌染色体。
红/ ET重组增加了CRISPR / Cas9的功效以扩展基因组研究。作为欧盟资助项目AgedBrainSYSBIO的一部分，我们制定了一项新的人类化大型基因组区域战略。通过将CRISPR / Cas9与Red / ET Recombineering组合，我们成功地将33kb人片段敲入小鼠ApoE基因座。
4-Way Recombineering（4WR）提供了一种新的快速简化的方法，可在单个体内重组步骤中从多4个DNA片段（例如PCR产物）组装质粒。4WR是Gene Bridges广受欢迎的直接克隆 -大肠杆菌菌株GB05-dir（目录号K008）的新应用。
Gene Bridges拥有Red / ET Recombination的专有权，并被允许颁发技术许可并提供重组工程服务。没有其他学术或商业组织有权提供重组工程服务。
genebridges2019年价格表
| 货号 | 品名 | 规格 | 价格 |
| K001 | *Quick&EasyBACModificationKit | Kit | 13900 |
| K002 | *CounterSelectionBACModificationKit | Kit | 16400 |
| K003 | *BACSubcloningKit | Kit | 13900 |
| K004 | *Quick&EasyConditionalKnockOutKit-FRT | Kit | 13900 |
| K005 | *Quick&EasyConditionalKnockOutKit-loxP | Kit | 13900 |
| K006 | *Quick&EasyE.ColiGeneDeletionKit | Kit | 13900 |
| K008 | *Direct-cloning-proficientE.colistrainGB05-dir | Kit | 12400 |
| K009 | *Recombineering-proficientE.colistrainGB08-red | Kit | 12400 |
| A001 | Pro-andEukaryoticNeomycinSelectionCassette(PGK-gb2-neo) | 50ng/ | 5980 |
| A002 | FRTflanked,ProandEuk.NeomycinSelect.Cassette(FRT-PGK-gb2-neo-FRT) | 50ng/ | 5980 |
| A003 | loxPflanked,ProandEuk.NeomycinSelect.Cassette(loxP-PGK-gb2-neo-loxP) | 50ng/ | 5980 |
| A004 | FRTflanked,ProandEuk.NeomycinSelect.CassetteplusloxPsite(FRT-PGK-gb2-neo-FRT-loxP) | 50ng/ | 5980 |
| A005 | FRTflanked,ProandEuk.NeomycinSelect.CassetteplusloxPsite2ndversion(loxP-FRT-PGK-gb2-neo-FRT) | 50ng/ | 5980 |
| A006 | FRTflankedChloramphenicolSelect.Cassette(FRT-cm-FRT) | 50ng/ | 5980 |
| A007 | loxPflankedChloramphenicolSelect.Cassette(loxP-cm-loxP) | 50ng/ | 5980 |
| A008 | FRTflankedAmpicillinSelect.Cassette(FRT-amp-FRT) | 50ng/ | 5980 |
| A009 | loxPflankedAmpicillinSelect.Cassette(loxP-amp-loxP) | 50ng/ | 5980 |
| A010 | FRTflanked,ProandEuk.HygromycinSelect.Cassette(FRT-PGK-gb2-hygro-FRT) | 50ng/ | 5980 |
| A011 | loxPflanked,ProandEuk.HygromycinSelect.Cassette(loxP-PGK-gb2-hygro-loxP) | 50ng/ | 5980 |
| A012 | iCre-FRT-neo-FRT(codonimprovedCre(iCre)withattachedFRTflanked,Pro-andEukaryoticNeomycinselectioncassette | 50ng/ | 6500 |
| A013 | iCreERT2-FRT-neo-FRT(ligand-inducibleiCrewithattachedFRTflanked,Pro-andEukaryoticNeomycinSelectioncassette) | 50ng/ | 6500 |
| A104 | EnhancedFlpExpressionPlasmid:707-FLPe;tet | 0.2ug/ | 5980 |
| A105 | EnhancedFlpExpressionPlasmid:708-FLPe;cm | 0.2ug/ | 5980 |
| A106 | EnhancedFlpExpressionPlasmid:709-FLPe;amp | 0.2ug/ | 5980 |
| A107 | EnhancedFlpExpressionPlasmid:710-FLPe;km | 0.2ug/ | 5980 |
| A112 | CreExpressionPlasmid:705-Cre;cm | 0.2ug/ | 5980 |
| A113 | CreExpressionPlasmid:706-Cre;tet | 0.2ug/ | 5980 |
| A114 | CreExpressionPlasmid:704-Cre;amp | 0.2ug/ | 5980 |
| A201 | EnhancedEukaryoticFLPExpressionPlasmid:pCAGGS-FLPe(Academia) | 0.2ug/ | 7500 |
| A203 | EukaryoticFLPoRecombinaseExpressionPlasmid:pCAGGS-FLPo(Academia)* | 0.2ug/ | 7500 |
| A204 | EukaryoticCreRecombinaseExpressionPlasmid:pCAGGS-Cre(Academia)* | 0.2ug/ | 7500 |
| A205 | EukaryoticDerRecombinaseExpressionPlasmid:CAGGS-Dre(Academia)* | 0.2ug/ | 7500 |
| A301 | Arabinose-inducibleProkaryoticCreRecombinaseExpressionPlasmid:pSC101-BAD-Cre | 0.2ug/ | 5980 |
| A302 | Arabinose-inducibleProkaryoticDreRecombinaseExpressionPlasmid:pSC101-BAD-Dre* | 0.2ug/ | 5980 |
| A303 | Arabinose-inducibleProkaryoticFLPeRecombinaseExpressionPlasmid:pSC101-BAD-FLPe(tet) | 0.2ug/ | 5980 |
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