BD Pharmingen- FITC Mouse Anti-Human CD51-CD61_BD Pharmingen
产品信息
荧光素标记
FITC
抗原名称
CD51/CD61 (Integrin αVβ3)
宿主
Mouse BALB/c IgG1, κ
免疫原
Human Osteoclastomas
简单描述
The 23C6 monoclonal antibody specifically binds to the Integrin αvβ3 heterodimeric complex (CD51/CD61) that is expressed on osteoclasts, endothelial cells, macrophages, melanoma cells, some B cells and in very low amounts on platelets. This antibody has been reported to crossreact with chicken, bovine, and rabbit, but not with rat αvβ3. The integrin αvβ3 heterodimer, often referred to as the vitronectin receptor, binds several ligands in addition to vitronectin including, von Willebrand factor, thrombospondin, osteopontin, bone sialoprotein, neural adhesion molecule L1, laminin, fibronectin, and fibrinogen. The binding of some ligands to the vitronectin receptor has been reported to be inhibited by the 23C6 monoclonal antibody.
商品描述
23C6
The 23C6 monoclonal antibody specifically binds to the Integrin αvβ3 heterodimeric complex (CD51/CD61) that is expressed on osteoclasts, endothelial cells, macrophages, melanoma cells, some B cells and in very low amounts on platelets. This antibody has been reported to crossreact with chicken, bovine, and rabbit, but not with rat αvβ3. The integrin αvβ3 heterodimer, often referred to as the vitronectin receptor, binds several ligands in addition to vitronectin including, von Willebrand factor, thrombospondin, osteopontin, bone sialoprotein, neural adhesion molecule L1, laminin, fibronectin, and fibrinogen. The binding of some ligands to the vitronectin receptor has been reported to be inhibited by the 23C6 monoclonal antibody.
同种型
Mouse BALB/c IgG1, κ
克隆号
克隆 23C6 (RUO)
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
20 µl
反应种属
Human (QC Testing), Chicken, Rabbit, Cow (Reported)
目标/特异性
CD51/CD61 (Integrin αVβ3)
背景
别名
Vitronectin Receptor; Itgav/Itgb3; Integrins alpha V, beta 3
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(9)
1. Athanasou NA, Quinn J, Horton MA, McGee JO. New sites of cellular vitronectin receptor immunoreactivity detected with osteoclast-reacting monoclonal antibodies 13C2 and 23C6. Bone Miner. 1990; 8(1):7-22. (Biology).
2. Chuntharapai A, Bodary S, Horton M, Kim KJ. Blocking monoclonal antibodies to alpha V beta 3 integrin: a unique epitope of alpha V beta 3 integrin is present on human osteoclasts. Exp Cell Res. 1993; 205(2):345-352. (Biology).
3. Davies J, Warwick J, Totty N, Philp R, Helfrich M, Horton M. The osteoclast functional antigen, implicated in the regulation of bone resorption, is biochemically related to the vitronectin receptor. J Cell Biol. 1989; 109(4):1817-1826. (Biology).
4. Horton MA, Lewis D, McNulty K, Pringle JA, Chambers TJ. Monoclonal antibodies to osteoclastomas (giant cell bone tumors): definition of osteoclast-specific cellular antigens. Cancer Res. 1985; 45(11):5663-5669. (Biology).
5. Horton MA, Taylor ML, Arnett TR, Helfrich MH. Arg-Gly-Asp (RGD) peptides and the anti-vitronectin receptor antibody 23C6 inhibit dentine resorption and cell spreading by osteoclasts. Exp Cell Res. 1991; 195(2):368-375. (Biology).
6. Nesbitt S, Nesbit A, Helfrich M, Horton M. Biochemical characterization of human osteoclast integrins. Osteoclasts express alpha v beta 3, alpha 2 beta 1, and alpha v beta 1 integrins. J Biol Chem. 1993; 268(22):16737-16745. (Biology).
7. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
8. Usatyuk PV, Parinandi NL, Natarajan V.. J Biol Chem. 2006; 281(46):35554-35566. (Biology).
9. Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007.
参考图片
Flow cytometric analysis of CD51/CD61 expression on human melanoma cell line M21. M21 cells were stained with either FITC Mouse IgG1, κ Isotype Control (Cat. No. 555748; dashed line histogram) or FITC Mouse Anti-Human CD51/CD61 (Cat. No. 555505; solid line histogram). Fluorescent histograms depicting CD51/CD61 (or Ig isotype) expression were derived from gated events with the side and forward light-scatter characteristics of viable cells. Flow cytometry was performed on a BD FACScan™ system.
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