BD Pharmingen- PE Mouse Anti-Human CD53_BD Pharmingen
产品信息
荧光素标记
PE
抗原名称
CD53
宿主
Mouse IgG1, κ
免疫原
Human Peripheral Blood Mononuclear Cells
简单描述
The HI29 monoclonal antibody specifically recognizes CD53. CD53 is a 35-42 kDa type III transmembrane glycoprotein that is expressed on all leucocytes, including plasma cells, but not on platelets, erythrocytes, and non-hematopoietic tissues. CD53 mediates signal transduction in monocytes and B cells. It has the broadest reactivity of all pan-leukocyte reagents used in immunohistology applications.
商品描述
HI29
The HI29 monoclonal antibody specifically recognizes CD53. CD53 is a 35-42 kDa type III transmembrane glycoprotein that is expressed on all leucocytes, including plasma cells, but not on platelets, erythrocytes, and non-hematopoietic tissues. CD53 mediates signal transduction in monocytes and B cells. It has the broadest reactivity of all pan-leukocyte reagents used in immunohistology applications.
同种型
Mouse IgG1, κ
克隆号
克隆 HI29 (RUO)
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
20 µl
反应种属
Human (QC Testing)
目标/特异性
CD53
背景
别名
tetraspanin-25; TSPAN25; tspan-25; MOX44
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(3)
1. Engel P, Wagner N, Tedder TF. CD86 Workshop Report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:703-705.
2. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
3. Olweus J, Lund-Johansen F, Horejsi V. CD53, a protein with four membrane-spanning domains, mediates signal transduction in human monocytes and B cells. J Immunol. 1993; 151(2):707-716. (Biology).
参考图片
Flow cytometric analysis of CD53 expression on human peripheral blood lymphocytes. Whole blood was stained with either PE Mouse Anti-Human CD53 (Cat. No. 555508; solid line histogram) or PE Mouse IgG1, κ Isotype Control (Cat. No. 555749; dashed line histogram). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Fluorescence histograms were derived from gated events with the side and forward light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™ system.
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