BD Pharmingen- Purified Mouse Anti-Human CD20_BD Pharmingen
产品信息
抗原名称
CD20
宿主
Mouse C57BL/6 IgG2b, κ
免疫原
Human 6.16c1.3 B cell line
简单描述
The 2H7 monoclonal antibody specifically binds to CD20, encoded by the
MS4A1
(Membrane-spanning 4-domains, subfamily A, member 1) gene. CD20 is a 33-37 kDa, unglycosylated four-transmembrane phosphoprotein. CD20 is expressed on pre-B-cells, resting and activated B cells, and follicular dendritic cells, but not plasma cells. Low level CD20 expression is observed on a small subset of normal circulating T lymphocytes. The CD20 molecule is involved in the regulation of B-cell activation.
商品描述
2H7
The 2H7 monoclonal antibody specifically binds to CD20, encoded by the
MS4A1
(Membrane-spanning 4-domains, subfamily A, member 1) gene. CD20 is a 33-37 kDa, unglycosylated four-transmembrane phosphoprotein. CD20 is expressed on pre-B-cells, resting and activated B cells, and follicular dendritic cells, but not plasma cells. Low level CD20 expression is observed on a small subset of normal circulating T lymphocytes. The CD20 molecule is involved in the regulation of B-cell activation.
同种型
Mouse C57BL/6 IgG2b, κ
克隆号
克隆 2H7 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development)
反应种属
Human (QC Testing), Rhesus,Cynomolgus,Baboon (Tested in Development)
目标/特异性
CD20
背景
别名
MS4A1; B1; Bp35; LEU-16; S7
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(3)
1. Hultin LE, Hausner MA, Hultin PM, Giorgi JV. CD20 (pan-B cell) antigen is expressed at a low level on a subpopulation of human T lymphocytes. Cytometry. 1993; 14(2):193-204. (Biology).
2. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
3. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
参考图片
Flow cytometric analysis of CD20 expression on human peripheral blood lymphocytes. Whole blood was stained with either Purified Mouse Anti-Human CD20 (Cat. No. 555621, solid line histogram) or Purified Mouse IgG2b κ Isotype Control (Cat. No. 555740, dashed line histogram), followed by FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555899). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Flouresence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphocytes.
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