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BD Pharmingen- APC Mouse Anti-Human CD86_BD Pharmingen

艾美捷科技 • 2025年08月26日 15:08:51 • 抗体 • 阅读 35 • 标签
产品信息
荧光素标记
APC
抗原名称
CD86 (B7-2)
宿主
Mouse BALB/c IgG1, κ
免疫原
Human HBL-1 Cell Line
简单描述
The 2331 (FUN-1) monoclonal antibody specifically recognizes a 75 kDa transmembrane cell surface protein, CD86 (B70/B7-2), expressed primarily on monocytes, dendritic cells and activated B cells. Competitive binding assays demonstrate that, while both 2331 (FUN-1) and IT2.2 (Anti-CD86) antibodies specifically recognize the same molecule, they react with different epitopes. CD86 is a ligand for CD28 and CTLA-4 and plays an important role in costimulation of T cells in primary immune response. The 2331 (FUN-1) antibody blocks the costimulatory activity of CD86 when tested in functional studies.
商品描述
2331 (FUN-1) The 2331 (FUN-1) monoclonal antibody specifically recognizes a 75 kDa transmembrane cell surface protein, CD86 (B70/B7-2), expressed primarily on monocytes, dendritic cells and activated B cells. Competitive binding assays demonstrate that, while both 2331 (FUN-1) and IT2.2 (Anti-CD86) antibodies specifically recognize the same molecule, they react with different epitopes. CD86 is a ligand for CD28 and CTLA-4 and plays an important role in costimulation of T cells in primary immune response. The 2331 (FUN-1) antibody blocks the costimulatory activity of CD86 when tested in functional studies.
同种型
Mouse BALB/c IgG1, κ
克隆号
克隆 2331 (FUN-1) (RUO)
产品详情
APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
APC
Red 627-640 nm
651 nm
660 nm
应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
20 µl
反应种属
Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
目标/特异性
CD86 (B7-2)
背景
别名
B7.2; B7-2; B-lymphocyte activation antigen B7-2; B70; BU63; CD28LG2; LAB72
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
数据库链接
Entrez-Gene ID
942

参考图片

Flow cytometric analysis of CD86 expression on Daudi cell line. Daudi cells were stained with either APC Mouse Anti-Human CD86 (Cat. No. 555660/560956; solid line histogram) or APC Mouse IgG1 κ Isotype Control (Cat. No. 555751; dashed line histogram). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells. Flow cytometry was carried out on a BD FACScan™ system.

Flow cytometric analysis of CD86 expression on Daudi cell line. Daudi cells were stained with either APC Mouse Anti-Human CD86 (Cat. No. 555660/560956; solid line histogram) or APC Mouse IgG1 κ Isotype Control (Cat. No. 555751; dashed line histogram). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells. Flow cytometry was carried out on a BD FACScan™ system.

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