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BD Pharmingen- Purified Mouse Anti-Human CD144_BD Pharmingen

产品信息
抗原名称
CD144
宿主
Mouse IgG1, κ
简单描述
The 55-7H1 antibody monoclonal antibody specifically binds to a calcium-independent epitope on Cadherin-5, a member of the cadherin family of calcium-dependent adhesion molecules. Cadherin-5 is also known as CD144 or VE-Cadherin. CD144 is expressed on endothelial cells in vivo and in vitro. It may play a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium.
商品描述
55-7H1 The 55-7H1 antibody monoclonal antibody specifically binds to a calcium-independent epitope on Cadherin-5, a member of the cadherin family of calcium-dependent adhesion molecules. Cadherin-5 is also known as CD144 or VE-Cadherin. CD144 is expressed on endothelial cells in vivo and in vitro. It may play a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium.
同种型
Mouse IgG1, κ
克隆号
克隆 55-7H1 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested), ELISA, Fluorescence microscopy, Immunohistochemistry-frozen, Immunoprecipitation (Tested During Development)
反应种属
Human (QC Testing)
目标/特异性
CD144 (VE-Cadherin)
背景
别名
VE-cadherin; Cadherin-5; CDH5; Vascular endothelial cadherin
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.

参考图片

Flow cytometric analysis of CD144 expression on Human Vein Endothelial Cells (HUVECs). HUVECs were stained with either Purified Mouse Anti-Human CD144 (Cat. No. 555661; solid line histogram) or Purified Mouse IgG1 κ Isotype Control (Cat. No. 550878), followed by PE Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 550589). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells.

Flow cytometric analysis of CD144 expression on Human Vein Endothelial Cells (HUVECs). HUVECs were stained with either Purified Mouse Anti-Human CD144 (Cat. No. 555661; solid line histogram) or Purified Mouse IgG1 κ Isotype Control (Cat. No. 550878), followed by PE Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 550589). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells.

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