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BD Pharmingen- FITC Mouse Anti-Human Light Chain _BD Pharmingen

艾美捷科技 • 2025年08月26日 16:38:12 • 抗体 • 阅读 88 • 标签
产品信息
荧光素标记
FITC
宿主
Mouse IgG1, κ
简单描述
The JDC-12 monoclonal antibody specifically binds to human immunoglobulin light chain, lambda (λ). It does not bind to immunoglobulin κ light chains or heavy chains.
商品描述
JDC-12 The JDC-12 monoclonal antibody specifically binds to human immunoglobulin light chain, lambda (λ). It does not bind to immunoglobulin κ light chains or heavy chains.
同种型
Mouse IgG1, κ
克隆号
克隆 JDC-12 (RUO)
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
20 µl
反应种属
Human (QC Testing)
目标/特异性
Ig, λ Light Chain
背景
别名
Ig λ; IGL@; IGL; Immunoglobulin lambda locus
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(2) 1. Odendahl M, Jacobi A, Hansen A, et al. Disturbed peripheral B lymphocyte homeostasis in systemic lupus erythematosus. J Immunol. 2000; 165(10):5970-5979. (Clone-specific: Flow cytometry). 2. Sembries S, Pahl H, Stilgenbauer S, Döhner H, Schriever F. Reduced expression of adhesion molecules and cell signaling receptors by chronic lymphocytic leukemia cells with 11q deletion.. Blood. 1999; 93(2):624-31. (Biology).

参考图片

Two color flow cytometric analysis of λ light chain expression on human peripheral blood lymphocytes. Whole blood was stained with FITC Mouse Anti-Human Light Chain λ (Cat. No. 555796/562053) and PE Mouse Anti-Human CD19 (Cat. No. 561741/555413). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The two-color dot plot depicting λ light chain versus CD19 expression was derived from gated events with the forward and side light-scattering characteristics of intact lymphocytes. Flow cytometry was performed on a BD FACScan™ system.

Two color flow cytometric analysis of λ light chain expression on human peripheral blood lymphocytes. Whole blood was stained with FITC Mouse Anti-Human Light Chain λ (Cat. No. 555796/562053) and PE Mouse Anti-Human CD19 (Cat. No. 561741/555413). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The two-color dot plot depicting λ light chain versus CD19 expression was derived from gated events with the forward and side light-scattering characteristics of intact lymphocytes. Flow cytometry was performed on a BD FACScan™ system.

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