BD Pharmingen- PE Mouse Anti-Human Light Chain- _BD Pharmingen
产品信息
荧光素标记
PE
宿主
Mouse IgG1, κ
简单描述
The JDC-12 monoclonal antibody specifically binds to human immunoglobulin light chain, lambda (λ). It does not bind to immunoglobulin κ light chains or heavy chains.
商品描述
JDC-12
The JDC-12 monoclonal antibody specifically binds to human immunoglobulin light chain, lambda (λ). It does not bind to immunoglobulin κ light chains or heavy chains.
同种型
Mouse IgG1, κ
克隆号
克隆 JDC-12 (RUO)
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
20 µl
反应种属
Human (QC Testing)
目标/特异性
Ig, λ Light Chain
背景
别名
Ig λ; IGL@; IGL; Immunoglobulin lambda locus
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(2)
1. Odendahl M, Jacobi A, Hansen A, et al. Disturbed peripheral B lymphocyte homeostasis in systemic lupus erythematosus. J Immunol. 2000; 165(10):5970-5979. (Clone-specific).
2. Sembries S, Pahl H, Stilgenbauer S, Döhner H, Schriever F. Reduced expression of adhesion molecules and cell signaling receptors by chronic lymphocytic leukemia cells with 11q deletion.. Blood. 1999; 93(2):624-31. (Clone-specific: Flow cytometry).
参考图片
Flow cytometric analysis of λ light chain expression on human peripheral blood lymphocytes. Whole blood was stained with PE Mouse Anti-Human Light Chain, λ (Cat. No. 555797/562054) and FITC Mouse Anti-Human CD19 (Cat. No. 555412/560994). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color fluorescent dot plot was derived from gated events with the forward and side light-scattering characteristics of intact lymphocytes. Flow cytometry was performed on a BD FACScan™ system.
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