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BD Pharmingen- PE Mouse Anti-Human CD134_BD Pharmingen

艾美捷科技 • 2025年08月26日 17:07:23 • 抗体 • 阅读 75 • 标签
产品信息
荧光素标记
PE
抗原名称
CD134
宿主
Mouse IgG1, κ
免疫原
Human HUT 102
简单描述
The ACT35 monoclonal antibody specifically binds to CD134 which is also known as OX40. The 35 kDa CD134 polypeptide is encoded by the TNFRSF4 gene. CD134 is a type I integral membrane glycoprotein and member of the tumor necrosis factor/nerve growth factor receptor (TNFR/NGFR) family. CD134 is expressed on activated T lymphocytes, hematopoietic precursor cells and fibroblasts. CD134 functions as a T cell costimulatory receptor when bound by OX40 Ligand/TNFSF4 that is expressed by antigen presenting cells. CD134 thereby plays roles in T-cell activation as well as the regulation of differentiation, proliferation or apoptosis of normal and malignant lymphoid cells. Analysis of the nucleotide sequence of the human TNFRSF4 cDNA reveals strong homology with the rat Tnfrsf4 cDNA sequence. OX40 was clustered as CD134 in the Sixth International Workshop on Human Leukocyte Differentiation Antigens.
商品描述
ACT35 The ACT35 monoclonal antibody specifically binds to CD134 which is also known as OX40. The 35 kDa CD134 polypeptide is encoded by the TNFRSF4 gene. CD134 is a type I integral membrane glycoprotein and member of the tumor necrosis factor/nerve growth factor receptor (TNFR/NGFR) family. CD134 is expressed on activated T lymphocytes, hematopoietic precursor cells and fibroblasts. CD134 functions as a T cell costimulatory receptor when bound by OX40 Ligand/TNFSF4 that is expressed by antigen presenting cells. CD134 thereby plays roles in T-cell activation as well as the regulation of differentiation, proliferation or apoptosis of normal and malignant lymphoid cells. Analysis of the nucleotide sequence of the human TNFRSF4 cDNA reveals strong homology with the rat Tnfrsf4 cDNA sequence. OX40 was clustered as CD134 in the Sixth International Workshop on Human Leukocyte Differentiation Antigens.
同种型
Mouse IgG1, κ
克隆号
克隆 ACT35 (also known as Ber-ACT35) (RUO)
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
20 µl
反应种属
Human (QC Testing)
目标/特异性
CD134 (OX40)
背景
别名
OX40; TNFRSF4; TXGP1L; OX40L Receptor
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(3) 1. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182. 2. Latza U, Durkop H, Schnittger S, et al. The human OX40 homolog: cDNA structure, expression and chromosomal assignment of the ACT35 antigen. Eur J Immunol. 1994; 24(3):677-683. (Biology). 3. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
数据库链接
Entrez-Gene ID
7293

参考图片

Flow cytometric profile of CD134 expression on PHA-stimulated peripheral blood lymphocytes. Human peripheral blood lymphocytes were stimulated with PHA and incubated with recombinant OX40 ligand (R&D Systems, Cat. No. 1054-ox), then stained with either PE Mouse Anti-Human CD134 (Cat. No. 555838, solid line histogram) or PE Mouse IgG1, κ Isotype Control (Cat. No. 555749, dashed line histogram). The fluorescence histograms depicting CD134 (or Ig isotype control) expression were derived from gated events with the side and light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™.

Flow cytometric profile of CD134 expression on non-stimulated peripheral blood lymphocytes. Human peripheral blood lymphocytes were stained with either PE Mouse Anti-Human CD134 (Cat. No. 555838, solid line histogram) or PE Mouse IgG1, κ Isotype Control (Cat. No. 555749, dashed line histogram). The fluorescence histograms depicting CD134 (or Ig isotype control) expression were derived from gated events with the side and light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™.

Flow cytometric profile of CD134 expression on PHA-stimulated peripheral blood lymphocytes. Human peripheral blood lymphocytes were stimulated with PHA and incubated with recombinant OX40 ligand (R&D Systems, Cat. No. 1054-ox), then stained with either PE Mouse Anti-Human CD134 (Cat. No. 555838, solid line histogram) or PE Mouse IgG1, κ Isotype Control (Cat. No. 555749, dashed line histogram). The fluorescence histograms depicting CD134 (or Ig isotype control) expression were derived from gated events with the side and light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™.

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