Interferon-γ (IFN-γ) is a potent multifunctional cytokine which is secreted by activated NK cells and CD4+TCRαβ+, CD8+TCRαβ+, and TCRγδ+ T cells and exerts its biological effects through specific binding to a single class of high affinity receptors. In addition to its antiviral effects, IFN-γ upregulates a number of lymphoid cell functions including the antimicrobial and antitumor responses of macrophages, NK cells, and neutrophils. In addition, IFN-γ can exert strong regulatory influences on the proliferation, differentiation, and effector responses of B cell and T cell subsets. These influences involve the action of  IFN-γ to boost MHC class I and II expression by antigen-presenting cells as well as direct effects on B cells and T cells themselves.  Recombinant rat IFN-γ (Cat. No. 550072) is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution and bovine serum albumin, with no preservatives.  Recombinant rat IFN-γ is ≥ 95% pure as determined by SDS-PAGE and an absorbance assay based on the Beers-Lambert law.  The endotoxin level is ≤ 0.1 ng/µg of rat IFN-γ, as measured in a chromegenic LAL assay.

(RUO)

100 µg/ml

ELISA Standard (Routinely Tested), Bioassay (Tested During Development)

Rat (QC Testing)

IFN-γ

Frozen aqueous buffered solution containing BSA.

Development References(3) 1. Farrar MA, Schreiber RD. The molecular cell biology of interferon-gamma and its receptor. Annu Rev Immunol. 1993; 11:571-611. (Biology). 2. Gray PW, Goeddel DV. Cloning and expression of murine immune interferon cDNA. Proc Natl Acad Sci U S A. 1983; 80(19):5842-5846. (Biology). 3. Green JA, Yeh TJ, Overall JC. Rapid, quantitative, semiautomated assay for virus-induced and immune human interferons. J Clin Microbiol. 1980; 12(3):433-438. (Biology).