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BD Pharmingen- PE Mouse Anti-Human MIP-1_BD Pharmingen

艾美捷科技 • 2025年08月19日 13:55:15 • 抗体 • 阅读 22 • 标签
产品信息
荧光素标记
PE
抗原名称
MIP-1β
宿主
Mouse IgG1, κ
免疫原
Recombinant Human MIP-1β
简单描述
The D21-1351 monoclonal antibody specifically binds to the human CC chemokine, MIP-1β (macrophage inflammatory protein-1β). Human MIP-1β shares approximately 75% homology with mouse MIP-1β at the amino acid level.  Expression of MIP-1β in human peripheral blood cells is induced by proinflammatory and mitogenic stimuli.  MIP-1β  is a chemoattractant for monocytes and lymphocytes. Human MIP-1β binds to receptors, CCR5 and CCR8. The human MIP-1β gene has been mapped to chromosome 17q11. The immunogen used to generate D21-1351 hybridoma was recombinant human MIP-1β.
商品描述
D21-1351 The D21-1351 monoclonal antibody specifically binds to the human CC chemokine, MIP-1β (macrophage inflammatory protein-1β). Human MIP-1β shares approximately 75% homology with mouse MIP-1β at the amino acid level.  Expression of MIP-1β in human peripheral blood cells is induced by proinflammatory and mitogenic stimuli.  MIP-1β  is a chemoattractant for monocytes and lymphocytes. Human MIP-1β binds to receptors, CCR5 and CCR8. The human MIP-1β gene has been mapped to chromosome 17q11. The immunogen used to generate D21-1351 hybridoma was recombinant human MIP-1β.
同种型
Mouse IgG1, κ
克隆号
克隆 D21-1351 (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Intracellular staining (flow cytometry) (Routinely Tested)
反应种属
Human (QC Testing)
目标/特异性
MIP-1β
背景
别名
Macrophage inflammatory protein 1-beta; CCL4; C-C motif chemokine 4; LAG-1
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(9) 1. Bernardini G, Hedrick J, Sozzani S. Identification of the CC chemokines TARC and macrophage inflammatory protein-1 beta as novel functional ligands for the CCR8 receptor. J Immunol. 1998; 28(2):582-588. (Biology). 2. Combadiere C, Ahuja SK, Tiffany HL, Murphy PM. Cloning and functional expression of CC CKR5, a human monocyte CC chemokine receptor selective for MIP-1(alpha), MIP-1(beta), and RANTES. J Leukoc Biol. 1996; 60(1):147-152. (Biology). 3. Lipes MA, Napolitano M, Jeang KT, Chang NT, Leonard WJ. Identification, cloning, and characterization of an immune activation gene. Proc Natl Acad Sci U S A. 1988; 85(24):9704-9708. (Biology). 4. Napolitano M, Seamon KB, Leonard WJ. Identification of cell surface receptors for the Act-2 cytokine. J Exp Med. 1990; 172(1):285-289. (Biology). 5. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology: Flow cytometry, IC/FCM Block). 6. Raport CJ, Gosling J, Schweickart VL, Gray PW, Charo IF. Molecular cloning and functional characterization of a novel human CC chemokine receptor (CCR5) for RANTES, MIP-1beta, and MIP-1alpha. J Biol Chem. 1996; 271(29):17161-17166. (Biology). 7. Samson M, Labbe O, Mollereau C, Vassart G, Parmentier M. Molecular cloning and functional expression of a new human CC-chemokine receptor gene. Biochemistry. 1996; 35(11):3362-3367. (Biology). 8. Sherry B, Tekamp-Olson P, Gallegos C. one of those components, macrophage inflammatory protein 1 beta. J Exp Med. 1988; 168(6):2251-2259. (Biology). 9. Ziegler SF, Tough TW, Franklin TL, Armitage RJ, Alderson MR. Induction of macrophage inflammatory protein-1 beta gene expression in human monocytes by lipopolysaccharide and IL-7. J Immunol. 1991; 147(7):2234-2239. (Biology).

参考图片

Expression of MIP-1β by stimulated human monocytes. Human PBMC were stimulated with human IFN-γ (20 ng/ml final concentration; Cat. No. 554616/554617) for one hour followed by overnight stimulation with LPS (1 µg/ml final concentration; Sigma, Cat. No. L-8272) in the presence of GolgiStop™ (2 µM final concentration; Cat. No. 554724). The PBMC were harvested, fixed, permeabilized, and stained with 0.03 µg of PE Mouse Anti-Human MIP-1β (Cat. 550078/561120; Left Panel) following Pharmingen's staining protocol. The data reflects gating on monocytes, based on forward and side scattered light signals. To demonstrate specificity of staining, binding by the PE Mouse Anti-Human MIP-1β antibody was blocked by preincubation with recombinant human MIP-1β (0.25 µg; Middle panel) and by preincubation of the fixed/permeabilized cells with excess unlabeled D21- 1351 antibody (5 µg; custom order; Right panel) prior to staining with PE Mouse Anti-Human MIP-1β. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control and verified using the unlabeled antibody blocking control.

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