BD Pharmingen- Purified Mouse Anti-Rat CD3_BD Pharmingen
产品信息
抗原名称
CD3
宿主
Mouse BALB/c IgG3, κ
免疫原
PHA-stimulated rat lymph node and spleen cells
简单描述
The G4.18 monoclonal antibody specifically recognizes the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes, peripheral T lymphocytes, and dendritic epidermal T cells. It has been reported that CD3 expression is down-regulated within 24 hours in concanavalin A-stimulated rat T cells, and soluble mAb inhibits the allogeneic mixed-lymphocyte proliferative response and cell-mediated cytotoxicity to allogeneic target cells. In vivo treatment with G4.18 mAb prevents cardiac and skin allograft rejection, resulting in donor-specific tolerance. Pre-incubation of splenocytes with the alternate anti-rat CD3 monoclonal antibody, 1F4, blocks staining with mAb G4.18.
商品描述
G4.18
The G4.18 monoclonal antibody specifically recognizes the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes, peripheral T lymphocytes, and dendritic epidermal T cells. It has been reported that CD3 expression is down-regulated within 24 hours in concanavalin A-stimulated rat T cells, and soluble mAb inhibits the allogeneic mixed-lymphocyte proliferative response and cell-mediated cytotoxicity to allogeneic target cells. In vivo treatment with G4.18 mAb prevents cardiac and skin allograft rejection, resulting in donor-specific tolerance. Pre-incubation of splenocytes with the alternate anti-rat CD3 monoclonal antibody, 1F4, blocks staining with mAb G4.18.
同种型
Mouse BALB/c IgG3, κ
克隆号
克隆 G4.18 (RUO)
浓度
31.25 µg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development), Cytotoxicity, Immunoprecipitation, Inhibition, Stimulation (Reported)
反应种属
Rat (QC Testing)
目标/特异性
CD3
背景
别名
CD3 Complex; T3
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, protein stabilizer, goat serum, and ≤ 0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, protein stabilizer, goat serum, and ≤ 0.09% sodium azide.
文献
文献
研发参考(3)
1. Naper C, Vaage JT, Lambracht D, et al. Alloreactive natural killer cells in the rat: complex genetics of major histocompatibility complex control. Eur J Immunol. 1995; 25(5):1249-1256. (Clone-specific: Cytotoxicity).
2. Nelson DJ, McMenamin C, McWilliam AS, Brenan M, Holt PG. Development of the airway intraepithelial dendritic cell network in the rat from class II major histocompatibility (Ia)-negative precursors: differential regulation of Ia expression at different levels of the respiratory tract. J Exp Med. 1994; 179(1):203-212. (Clone-specific: Immunohistochemistry).
3. Nicolls MR, Aversa GG, Pearce NW, et al. Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody.. Transplantation. 1993; 55(3):459-68. (Immunogen: Cytotoxicity, Flow cytometry, Immunohistochemistry, Immunoprecipitation, Inhibition, Stimulation).
参考图片
Immunohistochemical staining of T lymphocytes. The frozen section of normal rat spleen was reacted with Purified Mouse Anti-Rat CD3 (Cat. No. 550295). T lymphocytes in the periarteriolar sheath, are identified by the brown labeling of their cell surface membrane.
Immunohistochemical staining of T lymphocytes. The frozen section of normal rat spleen was reacted with Purified Mouse Anti-Rat CD3 (Cat. No. 550295). T lymphocytes in the periarteriolar sheath, are identified by the brown labeling of their cell surface membrane.
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