CD107b

Rat LEW x BN IgG1, κ

Mouse C57Bl/6 peritoneal exudate cells

The M3/84 monoclonal antibody specifically binds to CD107b which is also known as Mac-3, Lysosome-associated membrane protein 2 (LAMP-2/Lamp2/Lamp II), and Lysosomal membrane glycoprotein type B (LGP-B). CD107b is a single-pass type I transmembrane glycoprotein that constitutes a major integral membrane protein of lysosomes and may play a role in lysosomal function. CD107b is also expressed on the surface of mouse mononuclear phagocytes. Surface expression of the 92-110-kDa glycoprotein antigen increases during differentiation of monocytes to activated macrophages and may play a role in adhesion. The M3/84 mAb can detect CD107b antigen on tissue macrophages, thioglycollate-elicited peritoneal macrophages, and some myeloid cell lines, but not on lymphocytes or monocytes. In the bone marrow, very few cells display CD107b antigen on the surface, but a large proportion express cytoplasmic CD107b. The M3/84 antibody has also been reported to stain dendritic cells and endothelium in sections of thymus (both medulla and cortex), lymph nodes, spleen (white pulp), and gut-associated lymphoid tissue.

M3/84 The M3/84 monoclonal antibody specifically binds to CD107b which is also known as Mac-3, Lysosome-associated membrane protein 2 (LAMP-2/Lamp2/Lamp II), and Lysosomal membrane glycoprotein type B (LGP-B). CD107b is a single-pass type I transmembrane glycoprotein that constitutes a major integral membrane protein of lysosomes and may play a role in lysosomal function. CD107b is also expressed on the surface of mouse mononuclear phagocytes. Surface expression of the 92-110-kDa glycoprotein antigen increases during differentiation of monocytes to activated macrophages and may play a role in adhesion. The M3/84 mAb can detect CD107b antigen on tissue macrophages, thioglycollate-elicited peritoneal macrophages, and some myeloid cell lines, but not on lymphocytes or monocytes. In the bone marrow, very few cells display CD107b antigen on the surface, but a large proportion express cytoplasmic CD107b. The M3/84 antibody has also been reported to stain dendritic cells and endothelium in sections of thymus (both medulla and cortex), lymph nodes, spleen (white pulp), and gut-associated lymphoid tissue.

Rat LEW x BN IgG1, κ

克隆 M3/84 (RUO)

31.25 µg/ml

Flow cytometry (Routinely Tested), Immunohistochemistry-paraffin, Immunohistochemistry-zinc-fixed (Tested During Development), Immunohistochemistry-frozen, Immunoprecipitation (Reported)

Mouse (QC Testing)

CD107b (LAMP-2)

Mac-3; Lamp2; LAMP-2; Lysosome-associated membrane glycoprotein 2; LGP-B

Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.

研发参考(6) 1. Chen JW, Murphy TL, Willingham MC, Pastan I, August JT. Identification of two lysosomal membrane glycoproteins. J Cell Biol. 1985; 101(1):85-95. (Clone-specific: Immunoprecipitation). 2. Flotte TJ, Springer TA, Thorbecke GJ. Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets. Am J Pathol. 1983; 111(1):112-124. (Clone-specific: Immunohistochemistry). 3. Ho MK, Springer TA. Tissue distribution, structural characterization, and biosynthesis of Mac-3, a macrophage surface glycoprotein exhibiting molecular weight heterogeneity. J Biol Chem. 1983; 285(1):636-642. (Clone-specific: Immunoprecipitation). 4. Ralph P, Ho MK, Litcofsky PB, Springer TA. Expression and induction in vitro of macrophage differentiation antigens on murine cell lines. J Immunol. 1983; 130(1):108-114. (Clone-specific: Immunoprecipitation). 5. Springer TA. Monoclonal antibody analysis of complex biological systems. Combination of cell hybridization and immunoadsorbents in a novel cascade procedure and its application to the macrophage cell surface. J Biol Chem. 1981; 256(8):3833-3839. (Immunogen: Immunoprecipitation). 6. Walker EB, Akporiaye ET, Warner NL, Stewart CC. Characterization of subsets of bone marrow-derived macrophages by flow cytometry analysis. J Leukoc Biol. 1985; 37(2):121-136. (Biology).