BD Pharmingen- Purified Mouse Anti-Rat CD11b-c_BD Pharmingen
产品信息
抗原名称
CD11b/c
宿主
Mouse BALB/c IgG2a, κ
免疫原
Resident peritoneal cells from (PVG.RT1[c] x PVG.RT1[u]) and (PVG.RT1[c] x PVG.RT1[a]) F1-hybrid rat
简单描述
The OX-42 monoclonal antibody specifically binds to the CR3 complement (C3bi) receptor found on most monocytes, granulocytes, macrophages, dendritic cells, and microglia. It appears to recognize a common epitope shared by CD11b and CD11c (integrin αM and αX chains). OX-42 antibody inhibits C3bi binding activity.
商品描述
OX-42
The OX-42 monoclonal antibody specifically binds to the CR3 complement (C3bi) receptor found on most monocytes, granulocytes, macrophages, dendritic cells, and microglia. It appears to recognize a common epitope shared by CD11b and CD11c (integrin αM and αX chains). OX-42 antibody inhibits C3bi binding activity.
同种型
Mouse BALB/c IgG2a, κ
克隆号
克隆 OX-42 (RUO)
浓度
62.5 µg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
反应种属
Rat (QC Testing)
目标/特异性
CD11b,c
背景
别名
Itgam/Integrin, alpha M, C3bi receptor, CR3; Itgad/Integrin, alpha D
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
文献
文献
研发参考(6)
1. Issekutz AC, Issekutz TB. A major portion of polymorphonuclear leukocyte and T lymphocyte migration to arthritic joints in the rat is via LFA-1/MAC-1-independent mechanisms. Clin Immunol Immunopathol. 1993; 67(3):257-263. (Biology: Blocking).
2. Issekutz AC, Issekutz TB. The contribution of LFA-1 (CD11a/CD18) and MAC-1 (CD11b/CD18) to the in vivo migration of polymorphonuclear leucocytes to inflammatory reactions in the rat. Immunology. 1992; 76(4):655-661. (Biology: Blocking).
3. Robinson AP, White TM, Mason DW. MRC OX-43: a monoclonal antibody which reacts with all vascular endothelium in the rat except that of brain capillaries. Immunology. 1986; 57(2):231-237. (Immunogen).
4. Robinson AP, White TM, Mason DW. Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3. Immunology. 1986; 57(2):239-247. (Immunogen: Blocking, Immunoprecipitation).
5. Tamatani T, Kotani M, Miyasaka M. Characterization of the rat leukocyte integrin, CD11/CD18, by the use of LFA-1 subunit-specific monoclonal antibodies. Eur J Immunol. 1991; 21(3):627-633. (Biology: Immunoprecipitation).
6. Yamazaki T, Seko Y, Tamatani T, et al. Expression of intercellular adhesion molecule-1 in rat heart with ischemia/reperfusion and limitation of infarct size by treatment with antibodies against cell adhesion molecules. Am J Pathol. 1993; 143(2):410-418. (Biology: Blocking).
参考图片
Immunohistochemical staining of granulocytes and macrophages. A frozen section from rat spleen was stained with Mouse Anti-Rat CD11b/c (Cat.550299). Granulocytes and macrophages can be identified by the intense brown labeling of their cell surface membranes (40x magnification).
Immunohistochemical staining of granulocytes and macrophages. A frozen section from rat spleen was stained with Mouse Anti-Rat CD11b/c (Cat.550299). Granulocytes and macrophages can be identified by the intense brown labeling of their cell surface membranes (40x magnification).
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