Rat Monocyte Chemoattractant Protein (MCP-1) is a member of the C-C chemokine superfamily and a homolog of human MCP-1. MCP-1 is expressed by a variety of cell types including monocyte/macrophages, endothelial cells and mesangial cells.3 MCP-1 has chemoattractant activity for monocytes, lymphocytes and basophils but is not active for neutrophils. Recombinant rat MCP-1 is a highly glycosylated protein ranging in size from 27 - 30 kD as measured by SDS-PAGE analysis. Furthermore, there is evidence from experimental animal models that MCP-1 can suppress tumor formation by attracting monocytes to the tumor site.  Recombinant rat MCP-1 (MN 555110) is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution containing glycerol and 1.0 mg/ml bovine serum albumin, with no preservatives. Recombinant rat MCP-1 is ≥ 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law. The endotoxin level is ≤ 0.1 ng per µg of rat MCP-1, as measured in a chromogenic LAL assay.

(RUO)

0.1 mg/ml

ELISA Standard (Routinely Tested), Bioassay, Intracellular block/flow cytometry (Tested During Development)

Rat (QC Testing)

MCP-1

Frozen aqueous buffered solution containing BSA and glycerol.

Development References(5) 1. Capsoni F, Minonzio F, Ongari AM, Zanussi C. A new simplified single-filter assay for 'in vitro' evaluation of chemotaxis of 51Cr-labeled polymorphonuclear leukocytes. J Immunol Methods. 1989; 120(1):125-131. (Methodology). 2. Haelens A, Wuyts A, Proost P, Struyf S, Opdenakker G, van Damme J.. Leukocyte migration and activation by murine chemokines. Immunobiology. 1996; 195(4-5):499-521. (Biology). 3. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology). 4. Rollins BJ, Sunday ME. Suppression of tumor formation in vivo by expression of the JE gene in malignant cells.. Mol Cell Biol. 1991; 11(6):3125-3131. (Biology). 5. Yoshimura T, Takeya M, Takahashi K. Molecular cloning of rat monocyte chemoattractant protein-1 (MCP-1) and its expression in rat spleen cells and tumor cell lines. Biochem Biophys Res Commun. 1991; 174(2):504-509. (Biology).