BD Pharmingen- Biotin Rat Anti-Mouse IgG2a_BD Pharmingen
产品信息
宿主
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
免疫原
Pooled BALB/c and C57BL/6 mouse Ig
简单描述
The R19-15 antibody recognizes an epitope in the CH3 domain of mouse IgG2a, with strong reactivity to the
Igh-I[a]
allotype and weaker reactivity to
Igh-I[b]
. It does not react with other Ig isotypes. Molecular genetic analyses suggest that the
Igh-I[b]
allele, which encodes
IgG2a[b]
,
is derived from a locus found in several wild mouse subspecies, but not domestic mice, which encodes the IgG2c isotype.
商品描述
R19-15
The R19-15 antibody recognizes an epitope in the CH3 domain of mouse IgG2a, with strong reactivity to the
Igh-I[a]
allotype and weaker reactivity to
Igh-I[b]
. It does not react with other Ig isotypes. Molecular genetic analyses suggest that the
Igh-I[b]
allele, which encodes
IgG2a[b]
,
is derived from a locus found in several wild mouse subspecies, but not domestic mice, which encodes the IgG2c isotype.
同种型
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
克隆号
克隆 R19-15 (RUO)
浓度
250 µg/ml
产品详情
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
应用
实验应用
ELISA (Routinely Tested), Flow cytometry, Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development)
反应种属
Mouse (QC Testing)
目标/特异性
IgG2a
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
文献
文献
研发参考(2)
1. Martin RM, Silva A, Lew AM. The Igh-1 sequence of the non-obese diabetic (NOD) mouse assigns it to the IgG2c isotype. Immunogenetics. 1997; 46(2):167-168. (Biology).
2. Morgado MG, Cam P, Gris-Liebe C, Cazenave PA, Jouvin-Marche E. Further evidence that BALB/c and C57BL/6 gamma 2a genes originate from two distinct isotypes. EMBO J. 1989; 8(11):3245-3251. (Biology).
参考图片
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