BD Pharmingen- Biotin Rat Anti-Mouse IgG2b_BD Pharmingen
产品信息
宿主
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
免疫原
Pooled Mouse Ig
简单描述
The R12-3 antibody recognizes an epitope in the CH3 domain of mouse IgG2b of
Igh-C[a]
and
Igh-C[b]
haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with R12-3 mAb.
商品描述
R12-3
The R12-3 antibody recognizes an epitope in the CH3 domain of mouse IgG2b of
Igh-C[a]
and
Igh-C[b]
haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with R12-3 mAb.
同种型
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
克隆号
克隆 R12-3 (RUO)
浓度
250 µg/ml
产品详情
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
应用
实验应用
ELISA (Routinely Tested), Flow cytometry, Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development)
反应种属
Mouse (QC Testing)
目标/特异性
IgG2b
背景
别名
Igh-3; gamma2b; Immunoglobulin heavy constant gamma 2B
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
文献
文献
研发参考(1)
1. Wu K, Chen A, Tan P, Pan ZQ. The Nedd8-conjugated ROC1-CUL1 core ubiquitin ligase utilizes Nedd8 charged surface residues for efficient polyubiquitin chain assembly catalyzed by Cdc34.. J Biol Chem. 2002; 277(1):516-27. (Biology).
参考图片
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