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BD Pharmingen- Biotin Goat Anti-Rabbit IgG_BD Pharmingen

产品信息
宿主
Goat Ig
简单描述
BD Pharmingen™ Biotin Goat Anti-Rabbit IgG is intended to be a second-step reagent for the immunohistochemical staining of cells pre-stained with Rabbit IgG primary antibodies.   Biotin Anti-Rabbit IgG is reported to have minimal crossreactivity with human, mouse or rat serum proteins.
商品描述
Poly1272 BD Pharmingen™ Biotin Goat Anti-Rabbit IgG is intended to be a second-step reagent for the immunohistochemical staining of cells pre-stained with Rabbit IgG primary antibodies.   Biotin Anti-Rabbit IgG is reported to have minimal crossreactivity with human, mouse or rat serum proteins.
同种型
Goat Ig
克隆号
克隆 Polyclonal (RUO)
产品详情
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
应用
实验应用
Flow cytometry (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required) (Tested During Development)
反应种属
Rabbit (QC Testing)
目标/特异性
IgG
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.

参考图片

Immunohistochemical staining of a formalin-fixed paraffin-embedded tissue section from normal human colon. Tissue sections were treated with Retrievagen A (Cat. No. 550524) and stained with Purified Rabbit Anti-Human CD88 (Cat. No. 550733) followed by Biotin Goat Anti-Rabbit IgG (Cat. No. 550338) and Streptavidin-HRP (Cat. No. 550946). For optimal indirect immunohistochemical staining, this antibody should be titrated (1:10 to 1:50 dilution) and visualized via Streptavidin-HRP together with the DAB detection system (Cat. No. 550880).

Flow cytometric analysis of CD88 expression on human peripheral blood granulocytes. Whole blood was stained with either Purified Rabbit IgG Isotype Standard (Cat. No. 550875; dashed line histogram) or Purified Rabbit Anti-Human CD88 (solid line histogram). CD88 (or Ig isotype standard) expression were visualized with Biotin Goat Anti-Rabbit IgG and PE Streptavidin (Cat. No. 554657). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable granulocytes.

Immunohistochemical staining of a formalin-fixed paraffin-embedded tissue section from normal human colon. Tissue sections were treated with Retrievagen A (Cat. No. 550524) and stained with Purified Rabbit Anti-Human CD88 (Cat. No. 550733) followed by Biotin Goat Anti-Rabbit IgG (Cat. No. 550338) and Streptavidin-HRP (Cat. No. 550946).  For optimal indirect immunohistochemical staining, this antibody should be titrated (1:10 to 1:50 dilution) and visualized via Streptavidin-HRP together with the DAB detection system (Cat. No. 550880).

Flow cytometric analysis of CD88 expression on human peripheral blood granulocytes. Whole blood was stained with either Purified Rabbit IgG Isotype Standard (Cat. No. 550875; dashed line histogram) or Purified Rabbit Anti-Human CD88 (solid line histogram). CD88 (or Ig isotype standard) expression were visualized with Biotin Goat Anti-Rabbit IgG and PE Streptavidin (Cat. No. 554657). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable granulocytes.

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