BD Pharmingen- Biotin Goat Anti-Mouse Ig -Multiple Adsorption_BD Pharmingen
产品信息
宿主
Goat Ig
简单描述
This polyclonal antibody reacts with whole molecule mouse IgG (heavy and light chains) and may also react with the light chains of other mouse immunoglobulins. This antibody has been reported to be reactive in ELISA with mouse IgG, IgM, and IgA. In addition, low level cross-reactivity with rat and hamseter Ig has been reported. Investigators should note that reactivity with some mouse IgM antibodies can be weak. Minimal cross-reaction to rat, human, bovine, horse and rabbit non-immunoglobulin serum proteins are expected.
Note:
This reagent may have considerable cross-reactivity on human peripheral blood leukocytes, contributing to undesired background signal, when used with the flow cytometric application. As a result, this product is not recommended for immunofluorescent staining and flow cytometric analysis of human cells. For this application, investigators may wish to consider FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988) as an alternative.
For immunohistochemical staining, Biotin Goat Anti-Mouse Ig (Cat. No. 550337) is recommended.
商品描述
Poly1270
This polyclonal antibody reacts with whole molecule mouse IgG (heavy and light chains) and may also react with the light chains of other mouse immunoglobulins. This antibody has been reported to be reactive in ELISA with mouse IgG, IgM, and IgA. In addition, low level cross-reactivity with rat and hamseter Ig has been reported. Investigators should note that reactivity with some mouse IgM antibodies can be weak. Minimal cross-reaction to rat, human, bovine, horse and rabbit non-immunoglobulin serum proteins are expected.
Note:
This reagent may have considerable cross-reactivity on human peripheral blood leukocytes, contributing to undesired background signal, when used with the flow cytometric application. As a result, this product is not recommended for immunofluorescent staining and flow cytometric analysis of human cells. For this application, investigators may wish to consider FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988) as an alternative.
For immunohistochemical staining, Biotin Goat Anti-Mouse Ig (Cat. No. 550337) is recommended.
同种型
Goat Ig
克隆号
克隆 Polyclonal (RUO)
浓度
0.5 mg/ml
产品详情
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
应用
实验应用
ELISA (Routinely Tested), Flow cytometry (Tested During Development)
反应种属
Mouse (QC Testing)
目标/特异性
Ig
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
参考图片
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