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BD Pharmingen- PE Rat Anti-Mouse PIR-A-B_BD Pharmingen

艾美捷科技 • 2025年08月19日 14:39:19 • 抗体 • 阅读 32 • 标签
产品信息
荧光素标记
PE
抗原名称
PIR-A, B
宿主
Rat LEW, also known as Lewis IgG1, κ
免疫原
Recombinant mouse PIR-A1 extracellular domain peptide and the mouse myeloid cell line, WEH13
简单描述
The 6C1 monoclonal antibody specifically recognizes the common epitopes of PIR-A and PIR-B (Paired Immunoglobulin-liked Receptors) in all mouse strains tested (A/J, BALB/cJ, C3H, C57BL/6, DBA/1, DBA/2, NZB, and SJL).  PIR-A and PIR-B are type-I transmembrane glycoproteins containing six Ig-like domains.  There are multiple PIR-A proteins which are activating receptors by virtue of their intracellular association with the ITAM ( I mmunoreceptor T yrosine-based A ctivation M otif)- containing Fc receptor γ chain (FcRγ) in mast cells  and macrophages.  FcRγ expression is required for PIR-A cell-surface expression on dendritic cells, B cells, and myeloid lineages.  In contrast, PIR-B is an inhibitory receptor which contains various ITIMs ( I mmunoreceptor T yrosine-based I nhibitory M otifs) in its cytoplasmic domain.  These receptors are expressed on B cells, granulocytes, mast cells, dendritic cells, and monocytes/macrophages, but not on thymocytes, T lymphocytes, erythroid lineage cells, or NK cells.  The level of cell-surface expression of these receptors increases as a function of B-cell activation and myeloid- and B-lineage differentiation.  The 6C1 antibody immunoprecipitates molecules of 85- and 125-kDa, which correspond to PIRA and PIR-B, respectively.
商品描述
6C1 The 6C1 monoclonal antibody specifically recognizes the common epitopes of PIR-A and PIR-B (Paired Immunoglobulin-liked Receptors) in all mouse strains tested (A/J, BALB/cJ, C3H, C57BL/6, DBA/1, DBA/2, NZB, and SJL).  PIR-A and PIR-B are type-I transmembrane glycoproteins containing six Ig-like domains.  There are multiple PIR-A proteins which are activating receptors by virtue of their intracellular association with the ITAM ( I mmunoreceptor T yrosine-based A ctivation M otif)- containing Fc receptor γ chain (FcRγ) in mast cells  and macrophages.  FcRγ expression is required for PIR-A cell-surface expression on dendritic cells, B cells, and myeloid lineages.  In contrast, PIR-B is an inhibitory receptor which contains various ITIMs ( I mmunoreceptor T yrosine-based I nhibitory M otifs) in its cytoplasmic domain.  These receptors are expressed on B cells, granulocytes, mast cells, dendritic cells, and monocytes/macrophages, but not on thymocytes, T lymphocytes, erythroid lineage cells, or NK cells.  The level of cell-surface expression of these receptors increases as a function of B-cell activation and myeloid- and B-lineage differentiation.  The 6C1 antibody immunoprecipitates molecules of 85- and 125-kDa, which correspond to PIRA and PIR-B, respectively.
同种型
Rat LEW, also known as Lewis IgG1, κ
克隆号
克隆 6C1 (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
PIR-A, B
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(6) 1. Bléry M, Kubagawa H, Chen CC, Vély F, Cooper MD, Vivier E. The paired Ig-like receptor PIR-B is an inhibitory receptor that recruits the protein-tyrosine phosphatase SHP-1. Proc Natl Acad Sci U S A. 1998; 95(5):2446-2451. (Biology). 2. Chen CC, Hurez V, Brockenbrough JS, Kubagawa H, Cooper MD. Paternal monoallelic expression of the paired immunoglobulin-like receptors PIR-A and PIR-B. Proc Natl Acad Sci U S A. 1999; 96(12):6868-6872. (Clone-specific). 3. Kubagawa H, Burrows PD, Cooper MD. A novel pair of immunoglobulin-like receptors expressed by B cells and myeloid cells. Proc Natl Acad Sci U S A. 1997; 94(10):5261-5266. (Biology). 4. Kubagawa H, Chen CC, Ho LH. Biochemical nature and cellular distribution of the paired immunoglobulin-like receptors, PIR-A and PIR-B. J Exp Med. 1999; 189(2):309-318. (Immunogen). 5. Maeda A, Kurosaki M, Kurosaki T. Paired immunoglobulin-like receptor (PIR)-A is involved in activating mast cells through its association with Fc receptor gamma chain. J Exp Med. 1998; 188(5):991-995. (Biology). 6. Taylor LS, McVicar DW. Functional association of FcepsilonRIgamma with arginine(632) of paired immunoglobulin-like receptor (PIR)-A3 in murine macrophages. Blood. 1999; 94(5):1790-1796. (Biology).

参考图片

Two-color analysis of the expression of PIR-A/B on mouse spleen cells. BALB/c splenocytes were preincubated with Mouse BD Fc Block™ (Cat. no. 553141/553142) and simultaneously stained with PE- conjugated 6C1 (both panels) and either FITCconjugated 1D3 (anti-mouse CD19, Cat. no. 557398/553785, left panel) or FITC- conjugated M1/70 (anti-mouse CD11b, Cat. no. 557396/553310, right panel) monoclonal antibodies. Left panel demonstrates that the majority of B lymphocytes (CD19-positive cells) express intermediate levels of PIR- A/B, while the right panel shows that most myeloid cells (CD11b- or Mac-1-positive) express high levels of PIR-A/B. Flow cytometry was performed on a BD FACScan™ Flow Cytometry System. The quadrant positions are based upon analyses of isotype controls.

Two-color analysis of the expression of PIR-A/B on mouse spleen cells. BALB/c splenocytes were preincubated with Mouse BD Fc Block™ (Cat. no. 553141/553142) and simultaneously stained with PE- conjugated 6C1 (both panels) and either  FITCconjugated 1D3 (anti-mouse CD19, Cat. no. 557398/553785, left panel) or FITC- conjugated M1/70 (anti-mouse CD11b, Cat. no. 557396/553310, right panel) monoclonal antibodies. Left panel demonstrates that the majority of B lymphocytes (CD19-positive cells) express intermediate levels of PIR- A/B, while the right panel shows that most myeloid cells (CD11b- or Mac-1-positive) express high levels of PIR-A/B. Flow cytometry was performed on a BD FACScan™ Flow Cytometry System. The quadrant positions are based upon analyses of isotype  controls.

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