BD Pharmingen- Z-VAD-FMK- General Caspase Inhibitor_BD Pharmingen
参考图片
Flow cytometric analysis of apoptosis in Jurkat cells (Human T-cell leukemia; ATCC TIB-152). Jurkat cells were preincubated with the following: no inhibitor (upper left and bottom left panels), 20 µM Z-VAD-FMK (upper center and bottom center panels) or 20 µM negative control inhibitor (Z-FA-FMK, upper right and bottom right panels) (Cat. No. 550411) for 30 min, and then either left untreated (bottom row) or treated with 4 µM of campthothecin for 3 hr (top row). Following incubation, cells were collected and stained with PE Annexin V (Cat. No. 559763) to identify cells undergoing apoptosis. The results indicate that in campthothecin treated cells, approximately 42% of the cells were induced to undergo apoptosis and the use of the general caspase inhibitor Z-VAD-FMK reduced the level of apoptosis to that observed in the untreated control. Cells treated with Z-FA-FMK (Cat. No. 550411) showed similar results to the campthothecin treated cells without inhibitor, indicating that the negative control inhibitor did not attenuate apoptosis.
Flow cytometric analysis of apoptosis in Jurkat cells (Human T-cell leukemia; ATCC TIB-152). Jurkat cells were preincubated with the following: no inhibitor (upper left and bottom left panels), 20 µM Z-VAD-FMK (upper center and bottom center panels) or 20 µM negative control inhibitor (Z-FA-FMK, upper right and bottom right panels) (Cat. No. 550411) for 30 min, and then either left untreated (bottom row) or treated with 4 µM of campthothecin for 3 hr (top row). Following incubation, cells were collected and stained with PE Annexin V (Cat. No. 559763) to identify cells undergoing apoptosis. The results indicate that in campthothecin treated cells, approximately 42% of the cells were induced to undergo apoptosis and the use of the general caspase inhibitor Z-VAD-FMK reduced the level of apoptosis to that observed in the untreated control. Cells treated with Z-FA-FMK (Cat. No. 550411) showed similar results to the campthothecin treated cells without inhibitor, indicating that the negative control inhibitor did not attenuate apoptosis.
本文来自投稿，不代表本人立场，如若转载，请注明出处：http://www.iamyjet.com/kangti/8670.html