IRF-3

Mouse IgG1, κ

Human IRF-3 aa. 56-427 Recombinant Fusion Protein

The SL-12.1 monoclonal antibody specifically recognizes human Interferon regulatory factor 3 (IRF-3). Viral infection in mammals can lead to the induction of multiple pathways as part of the host defense mechanism. One of the major pathways activated is the JAK-STAT pathway by various interferons (IFNα and IFNβ). These IFNs exert their influence via transcriptional activation of specific target genes involved in antiviral defense, for example the chemokine ISG15 gene or the major histocompatibility complex class I and II molecules. These genes in turn are regulated by the JAK-STAT signaling pathway and through interferon regulatory factors (IRFs). IRFs are a family of transcription factors that possess a broad range of activities. IRF-3 is one of nine members which all share a common DNA binding domain which binds to an IFN stimulated response element (ISRE) found in the majority of IFN-inducible promoters. The IRF3 gene expresses a 50 kDa protein which is constitutively expressed in all tissues. The protein undergoes post-translational modification as well as dimerization and is translocated from the cytoplasm to the nucleus upon viral infection or exposure to dsRNA.

SL-12.1 The SL-12.1 monoclonal antibody specifically recognizes human Interferon regulatory factor 3 (IRF-3). Viral infection in mammals can lead to the induction of multiple pathways as part of the host defense mechanism. One of the major pathways activated is the JAK-STAT pathway by various interferons (IFNα and IFNβ). These IFNs exert their influence via transcriptional activation of specific target genes involved in antiviral defense, for example the chemokine ISG15 gene or the major histocompatibility complex class I and II molecules. These genes in turn are regulated by the JAK-STAT signaling pathway and through interferon regulatory factors (IRFs). IRFs are a family of transcription factors that possess a broad range of activities. IRF-3 is one of nine members which all share a common DNA binding domain which binds to an IFN stimulated response element (ISRE) found in the majority of IFN-inducible promoters. The IRF3 gene expresses a 50 kDa protein which is constitutively expressed in all tissues. The protein undergoes post-translational modification as well as dimerization and is translocated from the cytoplasm to the nucleus upon viral infection or exposure to dsRNA.

Mouse IgG1, κ

克隆 SL-12.1 (also known as SL-12) (RUO)

0.5 mg/ml

Western blot (Routinely Tested), Blocking, Immunofluorescence, Immunoprecipitation (Reported)

Human (QC Testing)

IRF-3

Interferon regulatory factor 3; IRF-3; IRF3; IIAE7

Aqueous buffered solution containing ≤0.09% sodium azide.

研发参考(8) 1. Au WC, Moore PA, Lowther W, Juang YT, Pitha PM. Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes. Proc Natl Acad Sci U S A. 1995; 92(25):11657-11661. (Biology). 2. Darnell JE Jr, Kerr IM, Stark GR. Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins. Science. 1994; 264(5164):1415-1421. (Biology). 3. Hiscott J, Pitha P, Genin P, et al. Triggering the interferon response: the role of IRF-3 transcription factor.. J Interferon Cytokine Res. 1999; 19(1):1-13. (Biology). 4. Loh JE, Chang CH, Fodor WL, Flavell RA. Dissection of the interferon gamma-MHC class II signal transduction pathway reveals that type I and type II interferon systems share common signalling component(s). EMBO J. 1992; 11(4):1351-1363. (Biology). 5. Mamane Y, Heylbroeck C, Genin P, et al. Interferon regulatory factors: the next generation. Gene. 1999; 237(1):1-14. (Biology). 6. Reich N, Evans B, Levy D, Fahey D, Knight E Jr, Darnell JE Jr. Interferon-induced transcription of a gene encoding a 15-kDa protein depends on an upstream enhancer element. Proc Natl Acad Sci U S A. 1987; 84(18):6394-6398. (Biology). 7. Ronco LV, Karpova AY, Vidal M, Howley PM. Human papillomavirus 16 E6 oncoprotein binds to interferon regulatory factor-3 and inhibits its transcriptional activity. Genes Dev. 1998; 12(13):2061-2072. (Clone-specific: Western blot). 8. Wathelet MG, Lin CH, Parekh BS, Ronco LV, Howley PM, Maniatis T. Virus infection induces the assembly of coordinately activated transcription factors on the IFN-beta enhancer in vivo. Mol Cell. 1998; 1(4):507-518. (Clone-specific: Blocking, Immunofluorescence, Immunoprecipitation, Western blot).