CD34

Mouse BALB/c IgG1, κ

CD34-mouse IgG2a fusion protein and canine myelomonocytic leukemia ML3

The 2E9 antibody reacts with CD34, an ~110 kDa glycoprotein on the surface of bone marrow-derived progenitors of hematopoietic and endothelial cells. In the bone marrow, 1-3% of cells are stained with 2E9 mAb; whereas peripheral blood leukocytes are not stained. Immunomagnetic depletion of lineage-committed leukocytes from bone marrow results in about three-fold enrichment of CD34+ cells. CD34+ hematopoietic progenitors may be mobilized to the peripheral blood treatment with recombinant canine granulocyte colony-stimulating factor and stem-cell factor. Furthermore, CD34 is expressed on some canine leukemias. In the mouse, CD34 is also expressed on high endothelial venules (HEV) of lymph nodes and, in this form, functions as a ligand for L-selectin. CD34 expression on HEV of dog lymph nodes has been demonstrated with polyclonal anti-CD34 antibody.

2E9 The 2E9 antibody reacts with CD34, an ~110 kDa glycoprotein on the surface of bone marrow-derived progenitors of hematopoietic and endothelial cells. In the bone marrow, 1-3% of cells are stained with 2E9 mAb; whereas peripheral blood leukocytes are not stained. Immunomagnetic depletion of lineage-committed leukocytes from bone marrow results in about three-fold enrichment of CD34+ cells. CD34+ hematopoietic progenitors may be mobilized to the peripheral blood treatment with recombinant canine granulocyte colony-stimulating factor and stem-cell factor. Furthermore, CD34 is expressed on some canine leukemias. In the mouse, CD34 is also expressed on high endothelial venules (HEV) of lymph nodes and, in this form, functions as a ligand for L-selectin. CD34 expression on HEV of dog lymph nodes has been demonstrated with polyclonal anti-CD34 antibody.

Mouse BALB/c IgG1, κ

克隆 2E9 (RUO)

0.5 mg/ml

Flow cytometry (Routinely Tested)

RANTES

gp 105-120

Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.

研发参考(10) 1. Baumheter S, Singer MS, Henzel W, et al. Binding of L-selectin to the vascular sialomucin CD34. Science. 1993; 262(5132):436-438. (Biology). 2. Bhattacharya V, McSweeney PA, Shi Q, et al. Enhanced endothelialization and microvessel formation in polyester grafts seeded with CD34(+) bone marrow cells. Blood. 2000; 95(2):581-585. (Biology). 3. Bruno B, Nash RA, Wallace PM, et al. CD34+ selected bone marrow grafts are radioprotective and establish mixed chimerism in dogs given high dose total body irradiation. Transplantation. 1999; 68(3):338-344. (Clone-specific). 4. Georges GE, Storb R, Bruno B, et al. Engraftment of DLA-haploidentical marrow with ex vivo expanded, retrovirally transduced cytotoxic T lymphocytes. Blood. 2001; 98(12):3447-3455. (Biology). 5. Goerner M, Horn PA, Peterson L, et al. Sustained multilineage gene persistence and expression in dogs transplanted with CD34(+) marrow cells transduced by RD114-pseudotype oncoretrovirus vectors. Blood. 2001; 98(7):2065-2070. (Clone-specific). 6. Hagglund HG, McSweeney PA, Mathioudakis G, et al. Ex vivo expansion of canine dendritic cells from CD34+ bone marrow progenitor cells. Transplantation. 2000; 70(10):1437-1442. (Clone-specific). 7. McSweeney PA, Rouleau KA, Storb R, et al. Canine CD34: cloning of the cDNA and evaluation of an antiserum to recombinant protein. Blood. 1996; 88(6):1992-2003. (Biology). 8. McSweeney PA, Rouleau KA, Wallace PM, et al. Characterization of monoclonal antibodies that recognize canine CD34. Blood. 1998; 91(6):1977-1986. (Immunogen). 9. Niemeyer GP, Hudson J, Bridgman R, Spano J, Nash RA, Lothrop CD. Isolation and characterization of canine hematopoietic progenitor cells. Exp Hematol. 2001; 29(6):686-693. (Biology). 10. Vernau W, Moore PF. An immunophenotypic study of canine leukemias and preliminary assessment of clonality by polymerase chain reaction. Vet Immunol Immunopathol. 1999; 69(2-4):145-164. (Biology).