The BD OptEIA™ Reagent Set B (Cat. No. 550534) is intended for use in BD OptEIA™ ELISA sets utilizing 0.1 M sodium carbonate coating buffer at pH 9.5.  Reagents are provided to be sufficient for use in ELISA for 20 plates (96-well).  Please refer to the BD OptEIA™  specific Technical Data Sheet and/or the Certificate of Analysis for determination of the correct coating buffer. When using BD OptEIA™ ELISA Sets utilizing 0.2 M sodium phosphate coating buffer at pH 6.5, investigators are encouraged to use BD OptEIA™ Reagent Set A (Cat. No. 550536). Substrate Reagent A (51-2606KZ) contains hydrogen peroxide in a buffered solution (should be clear and colorless). Substrate Reagent B (51-2607KZ) contains 3, 3', 5, 5' tetramethylbenzidine (TMB) in an organic solvent. Substrate Reagent B should be colorless to a very light amber. Wash Concentrate (20X) (51-9003739) contains concentrated detergent solution with ProClin™-150 as a preservative (should be clear and colorless). If the Wash Concentrate contains visible crystals or precipitate, warm to room temperature and mix gently until dissolved. Prepare a working concentration of wash buffer (1X) by diluting the required quantity of 20X Wash Concentrate with deionized or distilled water (e.g. to prepare 2.0 L of Wash Buffer, add 100 mL 20X Wash Concentrate to 1900 mL water). At least 500 mL Wash buffer (1X) should be prepared for one 96-well plate. Stop Solution (51-2608KZ) contains 1M phosphoric acid (should be clear and colorless). Assay Diluent (51-2641KC) contains animal serum in a buffered solution with ProClin™-150 as a preservative (should be clear and red in color).  Assay Diluent is designed to be used for (a) the blocking of ELISA plates, (b) performing serial dilutions of the recombinant standard or the experimental sample, and/or (c) for the preparation of the Working Detector outlined in BD OptEIA™ protocols. Coating Buffer (51-2713KC) contains 0.1 M sodium carbonate buffer, pH 9.5 (should be clear and colorless).

(RUO)

ELISA (Routinely Tested)

OptEIA Substrates