BD Pharmingen- Purified Rat Anti-Mouse CD29_BD Pharmingen
产品信息
抗原名称
CD29 (Integrin β1)
宿主
Rat IgG2a, κ
免疫原
Mouse endothelial cell line
简单描述
The 9EG7 antibody reacts with the 130-kDa integrin β1 chain (CD29). CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin α chains. With α1 through α6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with αv (CD51), it forms αvβ1 integrin. It also associates with the integrin α7 α8 and α9 chains in non-lymphoid tissues. As a result, CD29 has a broad tissue distribution, including lymphocytes, endothelia, smooth muscle, and epithelia. 9EG7 mAb has been shown to inhibit both the α6β1-mediated binding of lymphocytes to endothelial cells and the adhesion mediated by activated, but not unactivated, α4β1-integrin. The source of the immunogen was mouse lymph node-derived endothelial cell line TME.
商品描述
9EG7
The 9EG7 antibody reacts with the 130-kDa integrin β1 chain (CD29). CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin α chains. With α1 through α6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with αv (CD51), it forms αvβ1 integrin. It also associates with the integrin α7 α8 and α9 chains in non-lymphoid tissues. As a result, CD29 has a broad tissue distribution, including lymphocytes, endothelia, smooth muscle, and epithelia. 9EG7 mAb has been shown to inhibit both the α6β1-mediated binding of lymphocytes to endothelial cells and the adhesion mediated by activated, but not unactivated, α4β1-integrin. The source of the immunogen was mouse lymph node-derived endothelial cell line TME.
同种型
Rat IgG2a, κ
克隆号
克隆 9EG7 (RUO)
浓度
31.25 µg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
反应种属
Mouse (QC Testing)
目标/特异性
CD29 (Integrin β1)
背景
别名
Integrin β1 chain
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
文献
文献
研发参考(3)
1. Lenter M, Uhlig H, Hamann A, Jenö P, Imhof B, Vestweber D. A monoclonal antibody against an activation epitope on mouse integrin chain beta 1 blocks adhesion of lymphocytes to the endothelial integrin alpha 6 beta 1. Proc Natl Acad Sci U S A. 1993; 90(19):9051-9055. (Immunogen).
2. Mouse Genome Database (MGD), Mouse Genome Informatics, The Jackson Laboratory. B2m, beta-2 microglobulin. Available: http://www.informatics.jax.org 4/15/1998.
3. Springer TA. Adhesion receptors of the immune system. Nature. 1990; 346(6283):425-434. (Biology).
参考图片
Immunohistochemical staining of CD29 positive cells. Frozen sections of normal mouse spleen were reacted with the anti- CD29 antibody. Endothelial cells of the central arteriole and splenic sinuses can be identified by the brown labeling of their cell membranes. Amplification 20X.
Immunohistochemical staining of CD29 positive cells. Frozen sections of normal mouse spleen were reacted with the anti- CD29 antibody. Endothelial cells of the central arteriole and splenic sinuses can be identified by the brown labeling of their cell membranes. Amplification 20X.
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