BD Pharmingen- Purified Rat Anti-Mouse Flk-1_BD Pharmingen
产品信息
抗原名称
Flk-1
宿主
Rat WI, also known as Wistar (outbred) IgG2a, κ
免疫原
Mouse Flk-1 Recombinant Protein
简单描述
The Avas 12α1 monoclonal antibody specifically binds to fetal liver kinase 1 (Flk-1) which is also known as CD309. Flk-1 is a receptor protein tyrosine kinase which is closely related to CD117 (c-kit) and CD140a (PDGF Receptor α chain) of the immunoglobulin superfamily. Flk-1, also known as VEGF Receptor-2 (VEGF-R2 or VEGFR2), is a receptor for vascular endothelial growth factor (VEGF). It is expressed, at the mRNA and protein levels, on distinct sets of mesoderm during gastrulation and on endothelial cells in embryonic and adult tissues.
In vivo
and
in vitro
studies indicate that Flk-1 is required for the embryonic development of vascular endothelial and hematopoietic cells.
商品描述
Avas 12α1
The Avas 12α1 monoclonal antibody specifically binds to fetal liver kinase 1 (Flk-1) which is also known as CD309. Flk-1 is a receptor protein tyrosine kinase which is closely related to CD117 (c-kit) and CD140a (PDGF Receptor α chain) of the immunoglobulin superfamily. Flk-1, also known as VEGF Receptor-2 (VEGF-R2 or VEGFR2), is a receptor for vascular endothelial growth factor (VEGF). It is expressed, at the mRNA and protein levels, on distinct sets of mesoderm during gastrulation and on endothelial cells in embryonic and adult tissues.
In vivo
and
in vitro
studies indicate that Flk-1 is required for the embryonic development of vascular endothelial and hematopoietic cells.
同种型
Rat WI, also known as Wistar (outbred) IgG2a, κ
克隆号
克隆 Avas 12α1 (RUO)
浓度
31.25 µg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
反应种属
Mouse (QC Testing)
目标/特异性
CD309 (VEGFR2)
背景
别名
CD309; Fetal liver kinase 1; Kdr; VEGF Receptor-2; VEGFR-2; AVAS12;
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
文献
文献
研发参考(8)
1. Hanahan D. Signaling vascular morphogenesis and maintenance. Science. 1997; 277(5322):48-50. (Biology).
2. Kataoka H, Takakura N, Nishikawa S, et al. Expressions of PDGF receptor alpha, c-Kit and Flk1 genes clustering in mouse chromosome 5 define distinct subsets of nascent mesodermal cells.. Dev Growth Differ. 1997; 39(6):729-40. (Immunogen).
3. Millauer B, Wizigmann-Voos S, Schnurch H, et al. High affinity VEGF binding and developmental expression suggest Flk-1 as a major regulator of vasculogenesis and angiogenesis. Cell. 1993; 72(6):835-846. (Biology).
4. Nishikawa SI, Nishikawa S, Hirashima M, Matsuyoshi N, Kodama H. Progressive lineage analysis by cell sorting and culture identifies FLK1+VE-cadherin+ cells at a diverging point of endothelial and hemopoietic lineages. Development. 1998; 125(9):1747-1757. (Biology).
5. Nishikawa SI, Nishikawa S, Kawamoto H, et al. In vitro generation of lymphohematopoietic cells from endothelial cells purified from murine embryos. Immunity. 1998; 8(6):761-769. (Biology).
6. Quinn TP, Peters KG, De Vries C, Ferrara N, Williams LT. Fetal liver kinase 1 is a receptor for vascular endothelial growth factor and is selectively expressed in vascular endothelium. Proc Natl Acad Sci U S A. 1993; 90(16):7533-7537. (Biology).
7. Shalaby F, Ho J, Stanford WL, et al. A requirement for Flk1 in primitive and definitive hematopoiesis and vasculogenesis. Cell. 1997; 89(6):981-990. (Biology).
8. Shalaby F, Rossant J, Yamaguchi TP, et al. Failure of blood-island formation and vasculogenesis in Flk-1-deficient mice. Nature. 1995; 376(6535):62-66. (Biology).
参考图片
Immunohistochemical staining of mouse Flk-1. Frozen sections of normal mouse spleen were reacted with Purified Rat Anti-Mouse Flk-1 (Cat. No. 550549), then visualized with Biotin Goat Anti-Rat Ig (Cat. No. 559286), Streptavidin-HRP (Cat. No. 550946), and the DAB detection system (Cat. No. 550880). Endothelial cells expressing Flk-1 can be identified by the brown labeling of their cell membranes. Amplification 20X.
Immunohistochemical staining of mouse Flk-1. Frozen sections of normal mouse spleen were reacted with Purified Rat Anti-Mouse Flk-1 (Cat. No. 550549), then visualized with Biotin Goat Anti-Rat Ig (Cat. No. 559286), Streptavidin-HRP (Cat. No. 550946), and the DAB detection system (Cat. No. 550880). Endothelial cells expressing Flk-1 can be identified by the brown labeling of their cell membranes. Amplification 20X.
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