BD Pharmingen- Purified Rat Anti-Mouse CD144_BD Pharmingen
产品信息
抗原名称
CD144
宿主
Rat LEW, also known as Lewis IgG2a, κ
免疫原
Mouse VE-Cadherin-Ig Fusion
简单描述
The 11D4.1 antibody monoclonal antibody specifically binds to mouse CD144, also known as VE-cadherin. CD144 is a type I transmembrane protein and is a member of the cadherin superfamily. VE-cadherin is an endothelial cell-specific, homophilic adhesion molecule. It is concentrated at interendothelial cells contacts and is thought to be involved in the maintenance of cell layer integrity. In vitro and in vivo studies indicate that the 11D.4 mAb interferes with VE-cadherin-mediated intercellular adhesion.
商品描述
11D4.1
The 11D4.1 antibody monoclonal antibody specifically binds to mouse CD144, also known as VE-cadherin. CD144 is a type I transmembrane protein and is a member of the cadherin superfamily. VE-cadherin is an endothelial cell-specific, homophilic adhesion molecule. It is concentrated at interendothelial cells contacts and is thought to be involved in the maintenance of cell layer integrity. In vitro and in vivo studies indicate that the 11D.4 mAb interferes with VE-cadherin-mediated intercellular adhesion.
同种型
Rat LEW, also known as Lewis IgG2a, κ
克隆号
克隆 11D4.1 (RUO)
浓度
125 µg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
反应种属
Mouse (QC Testing)
目标/特异性
CD144 (VE-Cadherin)
背景
别名
Cdh5; Cadherin-5; CADH5; VE-cadherin; Vascular endothelial cadherin; 7B4
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
文献
文献
研发参考(3)
1. Breier G, Breviario F, Caveda L, et al. Molecular cloning and expression of murine vascular endothelial-cadherin in early stage development of cardiovascular system.. Blood. 1996; 87(2):630-41. (Biology: Immunohistochemistry).
2. Gotsch U, Borges E, Bosse R, et al. VE-cadherin antibody accelerates neutrophil recruitment in vivo. J Cell Sci. 1997; 110(5):583-588. (Immunogen: Blocking, Immunoprecipitation).
3. Lampugnani MG, Resnati M, Raiteri M, et al. A novel endothelial-specific membrane protein is a marker of cell-cell contacts.. J Cell Biol. 1992; 118(6):1511-22. (Biology).
参考图片
Immunohistochemical staining of CD144. Frozen sections of normal mouse small intestine were reacted with the anti-CD144 antibody. Cells expressing VE-Cadherin can be identified by the brown labeling of their cell membranes. Amplification 20X.
Immunohistochemical staining of CD144. Frozen sections of normal mouse small intestine were reacted with the anti-CD144 antibody. Cells expressing VE-Cadherin can be identified by the brown labeling of their cell membranes. Amplification 20X.
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