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BD Pharmingen- Purified Rabbit Anti-Human CD88_BD Pharmingen

艾美捷科技 • 2025年08月19日 16:03:21 • 抗体 • 阅读 49 • 标签
产品信息
抗原名称
CD88 (C5a Receptor)
宿主
Rabbit IgG
简单描述
The human C5a receptor (C5aR) is expressed on granulocytes, monocytes, macrophages mast cells human cell lines of myeloid origin, and certain cell types in several organs, including dendritic cells of secondary lymphoid organs, liver  hepatocytes, lung bronchial and alveolar epithelial cells, lung vascular smooth muscle, endothelial cells astrocytes, microglia, and fibroblast-like cells of the brain. The C85-2506 is a rabbit monoclonal antibody generated by fusion of splenocytes from rabbits immunized with human C5aR peptides and the rabbit fusion partner 240E-1.
商品描述
C85-2506 The human C5a receptor (C5aR) is expressed on granulocytes, monocytes, macrophages mast cells human cell lines of myeloid origin, and certain cell types in several organs, including dendritic cells of secondary lymphoid organs, liver  hepatocytes, lung bronchial and alveolar epithelial cells, lung vascular smooth muscle, endothelial cells astrocytes, microglia, and fibroblast-like cells of the brain. The C85-2506 is a rabbit monoclonal antibody generated by fusion of splenocytes from rabbits immunized with human C5aR peptides and the rabbit fusion partner 240E-1.
同种型
Rabbit IgG
克隆号
克隆 C85-2506 (RUO)
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen (Tested During Development)
反应种属
Human (QC Testing)
目标/特异性
CD88 (C5a Receptor)
背景
别名
C5a Receptor
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.

参考图片

Flow cytometric analysis of CD88 expression on human granulocytes. Whole blood was stained with either Purified Rabbit Anti-Human CD88 (solid line histogram) or Purified Rabbit IgG (Jackson ImmunoResearch Cat. No. 011-000-003; dashed line histogram) at 1 µg/test, followed by FITC Goat Anti-Rabbit IgG (Cat. No. 554020). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 349202). Fluorescent histograms depicting CD88 (or Ig isotype control) expression were derived from gated events with the side and forward light-scattering characteristics of viable granulocytes.

Immunohistochemistry analysis of CD88 expression on human tonsil paraffin sections. Dendritic cells (Left Panel) or granulocytes (Right Panel) from human tonsil sections were stained with Purified Rabbit Anti-Human CD88 (2.5 µg/ml; Cat. No. 550733), followed by Biotinylated Donkey Anti-Rabbit Ig (5 µg/ml; Jackson ImmunoResearch Cat. No. 711-065-152) and Streptavidin HRP (Cat. No. 550946).

Immunohistochemistry analysis of CD88 expression on human tonsil paraffin sections. Dendritic cells (Left Panel) or granulocytes (Right Panel) from human tonsil sections were stained with Purified Rabbit Anti-Human CD88 (2.5 µg/ml; Cat. No. 550733), followed by Biotinylated Donkey Anti-Rabbit Ig (5 µg/ml; Jackson ImmunoResearch Cat. No. 711-065-152) and Streptavidin HRP (Cat. No. 550946).

Flow cytometric analysis of CD88 expression on human granulocytes. Whole blood was stained with either Purified Rabbit Anti-Human CD88 (solid line histogram) or Purified Rabbit IgG (Jackson ImmunoResearch Cat. No. 011-000-003; dashed line histogram) at 1 µg/test, followed by FITC Goat Anti-Rabbit IgG (Cat. No. 554020). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 349202). Fluorescent histograms depicting CD88 (or Ig isotype control) expression were derived from gated events with the side and forward light-scattering characteristics of viable granulocytes.

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