BD Pharmingen- PE Rat Anti-Human IL-12R-2_BD Pharmingen
产品信息
荧光素标记
PE
抗原名称
CD212 (IL-12 Receptor β1)
宿主
Rat IgG2a, κ
免疫原
Human IL-12Rβ2 Recombinant Protein
简单描述
The 2B6 monoclonal antibody specifically recognizes the Interleukin 12 Receptor beta 2 subunit (IL-12Rβ2). IL-12Rβ2 serves as the signaling subunit that combines with CD212 (Interleukin 12 Receptor beta 1 subunit/ IL-12Rβ1) to form the functional receptor for IL-12. The IL-12Rβ2 is expressed on T cells and NK cells. IL-12R is a glycoprotein member of the hemopoietin receptor superfamily and is related to gp130, the granulocyte-colony stimulating factor (G-CSF) receptor and leukemia inhibitory factor (LIF). IL-12 is an immunomodulatory cytokine produced mainly by antigen presenting cells (APC) and plays a role in promoting Th1-type responses and cell mediated immunity. It stimulates proliferation of T cells and NK cells, enhances lytic activity of lymphokine activated (LAK) cells and cytolytic T cells (CTL).
商品描述
2B6/12beta2
The 2B6 monoclonal antibody specifically recognizes the Interleukin 12 Receptor beta 2 subunit (IL-12Rβ2). IL-12Rβ2 serves as the signaling subunit that combines with CD212 (Interleukin 12 Receptor beta 1 subunit/ IL-12Rβ1) to form the functional receptor for IL-12. The IL-12Rβ2 is expressed on T cells and NK cells. IL-12R is a glycoprotein member of the hemopoietin receptor superfamily and is related to gp130, the granulocyte-colony stimulating factor (G-CSF) receptor and leukemia inhibitory factor (LIF). IL-12 is an immunomodulatory cytokine produced mainly by antigen presenting cells (APC) and plays a role in promoting Th1-type responses and cell mediated immunity. It stimulates proliferation of T cells and NK cells, enhances lytic activity of lymphokine activated (LAK) cells and cytolytic T cells (CTL).
同种型
Rat IgG2a, κ
克隆号
克隆 2B6/12beta2 (RUO)
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested), Fluorescence microscopy (Reported)
推荐用量
20 µl
反应种属
Human (QC Testing)
目标/特异性
CD212 (IL-12 Receptor β1)
背景
别名
IL12RB2; Interleukin-12 receptor beta-2 chain
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(4)
1. Chizzonite R, Truitt T, Podlaski FJ, et al. IL-12: monoclonal antibodies specific for the 40-kDa subunit block receptor binding and biologic activity on activated human lymphoblasts. J Immunol. 1991; 147(5):1548-1556. (Biology).
2. Chua AO, Chizzonite R, Desai BB, et al. Expression cloning of a human IL-12 receptor component. A new member of the cytokine receptor superfamily with strong homology to gp130. J Immunol. 1994; 153(1):128-136. (Biology).
3. Presky DH, Minetti LJ, Gillessen S, et al. Analysis of the multiple interactions between IL-12 and the high affinity IL-12 receptor complex. J Immunol. 1998; 160(5):2174-2179. (Biology).
4. Wu CY, Warrier RR, Carvajal DM, et al. Biological function and distribution of human interleukin-12 receptor beta chain. Eur J Immunol. 1996; 26(2):345-350. (Biology).
参考图片
Profile of CD3-stimulated peripheral blood mononuclear cells (3 days) analyzed by flow cytometry.
Profile of CD3-stimulated peripheral blood mononuclear cells (3 days) analyzed by flow cytometry.
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