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BD Pharmingen- APC Rat Anti-Mouse IgG1_BD Pharmingen

艾美捷科技 • 2025年08月19日 16:37:39 • 抗体 • 阅读 8 • 标签
产品信息
荧光素标记
APC
宿主
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
免疫原
Mouse IgG1, κ Antibody
简单描述
The X56 monoclonal antibody specifically binds to mouse IgG1 of Igh-C[a] and Igh-C[b] haplotypes. It does not crossreact with other Ig isotypes.
商品描述
X56 The X56 monoclonal antibody specifically binds to mouse IgG1 of Igh-C[a] and Igh-C[b] haplotypes. It does not crossreact with other Ig isotypes.
同种型
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
克隆号
克隆 X56 (RUO)
浓度
0.2 mg/ml
产品详情
APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
APC
Red 627-640 nm
651 nm
660 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
IgG1
背景
别名
Igh-4; Ighg1; Immunoglobulin heavy constant gamma 1
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(3) 1. Hao Z, Duncan GS, Su YW, et al.. The E3 ubiquitin ligase Mule acts through the ATM-p53 axis to maintain B lymphocyte homeostasis. J Exp Med. 2012; 209(1):173-186. (Methodology: Flow cytometry). 2. Kita-Furuyama M, Nagayama Y, Pichurin P, McLachlan SM, Rapoport B, Eguchi K. Dendritic cells infected with adenovirus expressing the thyrotrophin receptor induce Graves' hyperthyroidism in BALB/c mice. Clin Exp Immunol. 2003; 131(2):234-240. (Methodology: Flow cytometry). 3. Vuong BQ, Lee M, Kabir S, et al. Specific recruitment of protein kinase A to the immunoglobulin locus regulates class-switch recombination.. Nat Immunol. 2009; 10(4):420-6. (Clone-specific: Flow cytometry).
数据库链接
Entrez-Gene ID
16017

参考图片

Flow cytometric analysis of mouse CD22.2 and rat CD45RA expression using APC Rat Anti-Mouse IgG1 antibody as a second step. Left Plot: CD22.2 expression on mouse splenocytes. BALB/c mouse splenic leucocytes were either not stained (control staining; dashed line histogram) or stained with Purified Mouse IgG1, κ Anti-Mouse CD22.2 antibody (solid line histogram). After washing, the cells were then stained with APC Rat Anti-Mouse IgG1 antibody (Cat. No. 550874) at 1 µg/test. The fluorescence histogram showing CD22.2 expression (or control staining) was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Right Plot: CD45RA expression on rat splenocytes. Rat splenic leucocytes were either not stained (control staining; dashed line histogram) or stained with Purified Mouse IgG1, κ Anti- Rat CD45RA antibody (Cat. No. 554882; solid line histogram). After washing, the cells were then stained with APC Rat Anti-Mouse IgG1 antibody at 1 µg/test. The fluorescence histogram showing CD45RA expression (or control staining) was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes.         Flow cytometry and data analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

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