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BD Pharmingen- Purified Mouse Anti-Human Bcl-2 w-Control_BD Pharmingen

艾美捷科技 • 2025年08月20日 09:01:05 • 抗体 • 阅读 57 • 标签
产品信息
抗原名称
Bcl-2
简单描述
Bcl-2 is considered to be novel among proto-oncogenes because it blocks apoptosis (programmed cell death) in many cell types. Apoptosis is an active form of cellular suicide that typically requires new RNA and protein synthesis and is associated with distinct morphological changes including cell shrinkage, cytoplasm membrane blebbing, nuclear fragmentation and DNA degradation. Because Bcl-2 blocks apoptosis it may contribute to tumorigenisis by prolonging cell survival, rather than by accelerating the rate of cell proliferation. Human Bcl-2 protein migrates at a molecular weight of ~26 kDa by SDS-PAGE. Bcl-2/100 recognizes a 26 kDa band representing human Bcl-2. Additional minor bands at 27-31 kDa and 18-21 kDa may also be visualized. The 27-31 kDa upper band may represent a larger isoform, whereas the 18-21 kDa lower band may be an internal translation or proteolytic product, therefore, a synthetic peptide corresponding to amino acids 41-54 (GAAPAPGIFSSQPG) of human Bcl-2 was used as immunogen. This peptide sequence is not conserved between human and mouse. Bcl-2/100 does not cross-react with mouse Bcl-2. For detection of mouse Bcl-2 refer to clone 3F11 (Cat. No. 554218), polyclonal rabbit anti-rat/mouse Bcl-2 antiserum (Cat. No. 554087), and polyclonal rabbit anti-mouse Bcl-2 antiserum (Cat. No. 554279).
克隆号
Bcl-2/100
BD化合物表
  • 描述
    数量/尺寸
    零件号
    EntrezGene ID
  • Purified Mouse Anti-Human Bcl-2
    50 µg (1 ea)
    51-6511GR
    N/A
  • Jurkat Cell Lysate
    50 µg (1 ea)
    51-16526N
    N/A
应用
实验应用
Western blot (Routinely Tested), Flow cytometry, Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen (Tested During Development)
反应种属
Human (QC Testing)
目标/特异性
Bcl-2

参考图片

Western blot analysis of Bcl-2 (right panel). Lysate from Jurkat cells was probed with clone Bcl-2/100 at concentrations of 1.0 (lane 1), 0.5 (lane 2), and 0.125 µg/ml (lane 3). Bcl-2 is identified as a band of ~26 kDa.

Profile of permeabilized lymphocytes analyzed on a FACScan (BDIS, San Jose, CA) (left panel). Cells were stained with anti-human Bcl-2 (clone Bcl-2/100) or a mouse IgG1 isotype (negative) control followed by FITC-conjugated second step.

Formalin-fixed, paraffin-embedded tissue sections stained for Bcl-2 expression using clone Bcl-2/100 and the SA-HRP method. (Left panel) Normal tonsil. (Right panel) Follicular lymphoma. In normal tonsil the mantle zone and intrafollicular region are stained; only occasional Bcl-2 positive cells are seen in the germinal center. In follicular lymphoma, tumor follicles are Bcl-2 positive.

Profile of permeabilized lymphocytes analyzed on a FACScan (BDIS, San Jose, CA) (left panel). Cells were stained with anti-human Bcl-2 (clone Bcl-2/100) or a mouse IgG1 isotype (negative) control followed by FITC-conjugated second step.

Western blot analysis of Bcl-2 (right panel). Lysate from Jurkat cells was probed with clone Bcl-2/100 at concentrations of 1.0 (lane 1), 0.5 (lane 2), and 0.125 µg/ml (lane 3). Bcl-2 is identified as a band of ~26 kDa.

Formalin-fixed, paraffin-embedded tissue sections stained for Bcl-2 expression using clone Bcl-2/100 and the SA-HRP method. (Left panel) Normal tonsil. (Right panel) Follicular lymphoma. In normal tonsil the mantle zone and intrafollicular region are stained; only occasional Bcl-2 positive cells are seen in the germinal center. In follicular lymphoma, tumor follicles are Bcl-2 positive.

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