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BD Pharmingen- Purified Mouse Anti-Rat CD24_BD Pharmingen

艾美捷科技 • 2025年08月20日 09:14:07 • 抗体 • 阅读 10 • 标签
产品信息
抗原名称
CD24
宿主
Mouse BALB/c IgM, κ
简单描述
The HIS50 monoclonal antibody specifically binds to CD24 (Heat-Stable Antigen, HSA or HsAg), a variably glycosylated GPI-anchored membrane protein. In the rat, Cd24 mRNA is found in a variety of epithelia, neural tissue, lymph node germinal centers and thymus. The HIS50 antibody detects CD24 on most B-lineage cells in the bone marrow, on most highly surface IgM (sIgM)-positive B lymphocytes in the blood and spleen, and on marginal-zone B cells in the spleen and lymph node. Variable levels of CD24 are detected on B cells that expresses low levels of sIgM and on follicular B lymphocytes. The HIS50 antibody does not detect CD24 on rat thymocytes nor peripheral T lymphocytes, NK cells, or granulocytes. The HIS50 antibody crossreacts with intracellular antigen(s) in human lymphocytes.
商品描述
HIS50 The HIS50 monoclonal antibody specifically binds to CD24 (Heat-Stable Antigen, HSA or HsAg), a variably glycosylated GPI-anchored membrane protein. In the rat, Cd24 mRNA is found in a variety of epithelia, neural tissue, lymph node germinal centers and thymus. The HIS50 antibody detects CD24 on most B-lineage cells in the bone marrow, on most highly surface IgM (sIgM)-positive B lymphocytes in the blood and spleen, and on marginal-zone B cells in the spleen and lymph node. Variable levels of CD24 are detected on B cells that expresses low levels of sIgM and on follicular B lymphocytes. The HIS50 antibody does not detect CD24 on rat thymocytes nor peripheral T lymphocytes, NK cells, or granulocytes. The HIS50 antibody crossreacts with intracellular antigen(s) in human lymphocytes.
同种型
Mouse BALB/c IgM, κ
克隆号
克隆 HIS50 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested), Immunofluorescence, Immunohistochemistry-frozen, Western blot (Reported)
反应种属
Rat (QC Testing)
目标/特异性
CD24
背景
别名
HSA; Heat-stable antigen; Nectadrin; Cd24a; Signal transducer CD24
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(3) 1. Akashi T, Shirasawa T, Hirokawa K.. Gene expression of CD24 core polypeptide molecule in normal rat tissues and human tumor cell lines.. Virchows Arch. 1994; 425(4):399-406. (Biology: Bioassay). 2. Hermans MH, Deenen GJ, De Boer N, Bo W, Kroese FG, Opstelten D. Expression of HIS50 Ag: a rat homologue of mouse heat-stable antigen and human CD24 on B lymphoid cells in the rat. Immunology. 1997; 90(1):14-22. (Immunogen: Immunofluorescence, Immunohistochemistry). 3. Pearce EI, Ammerlaan WA, Opstelten D.. Characterization of rat HSA/CD24 protein bearing the B lineage-restricted epitope recognized by MAb HIS50.. Hybridoma. 1996 February; 15(1):33-42. (Clone-specific: Western blot).

参考图片

Two-color analysis of CD24 expression on B-lineage cells in rat bone marrow. Cells flushed from Lewis femurs were stained with PE-conjugated anti-rat CD45R B-lymphocyte-specific mAb HIS24 (Cat. no. 554881) and either purified isotype control mAb G155-228 (Cat. no. 553472, left panel) or purified mAb HIS50 (right panel), followed by FITC-conjugated anti-mouse IgM mAb II/41 (Cat. no. 553437). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

Two-color analysis of CD24 expression on B-lineage cells in rat bone marrow. Cells flushed from Lewis femurs were stained with PE-conjugated anti-rat CD45R B-lymphocyte-specific mAb HIS24 (Cat. no. 554881) and either purified isotype control mAb G155-228 (Cat. no. 553472, left panel) or purified mAb HIS50 (right panel), followed by FITC-conjugated anti-mouse IgM mAb II/41 (Cat. no. 553437). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

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