BD Pharmingen- Purified Mouse Anti-Human Trop-2_BD Pharmingen
产品信息
抗原名称
Trop-2
宿主
Mouse BALB/c IgG1, κ
免疫原
Human BeWo (choriocarcinoma) Cell Line
简单描述
The 162-46 monoclonal antibody recognizes human Trop-2 which is also known as GA733-1. Trop-2 is encoded by
TACSTD2
(tumor-associated calcium signal transducer 2). It is a single-pass type-1 transmembrane glycoprotein. Trop-2 was originally identified to be expressed on human trophoblast and choriocarcinoma cell lines. Trop-2 is expressed in most human carcinomas. Trop-2 appears to be involved in the transduction of an intracellular calcium signal, although the exact physiological mechanism has yet to be elucidated.
商品描述
162-46
The 162-46 monoclonal antibody recognizes human Trop-2 which is also known as GA733-1. Trop-2 is encoded by
TACSTD2
(tumor-associated calcium signal transducer 2). It is a single-pass type-1 transmembrane glycoprotein. Trop-2 was originally identified to be expressed on human trophoblast and choriocarcinoma cell lines. Trop-2 is expressed in most human carcinomas. Trop-2 appears to be involved in the transduction of an intracellular calcium signal, although the exact physiological mechanism has yet to be elucidated.
同种型
Mouse BALB/c IgG1, κ
克隆号
克隆 162-46 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested), Immunohistochemistry (Reported)
反应种属
Human (QC Testing)
目标/特异性
Trop-2
背景
别名
GA733-1; TROP2; EGP-1; EGP1; GP50; M1S1; TACD2
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(4)
1. Bobos M, Kotoula V, Kaloutsi V, Karayannopoulou G, Papadimitriou CS, Kostopoulos I. Aberrant CCND1 copies and cyclin D1 mRNA expression do not result in the production of functional cyclin D1 protein in anaplastic large cell lymphoma. Histol Histopathol. 2009; 24(8):1035-1048. (Biology: Immunohistochemistry).
2. Fornaro M, Dell'Arciprete R, Stella M, et al. Cloning of the gene encoding Trop-2, a cell-surface glycoprotein expressed by human carcinomas. Int J Cancer. 1995; 62(5):610-618. (Biology).
3. Lipinski M, Parks DR, Rouse RV, Herzenberg LA. Human trophoblast cell-surface antigens defined by monoclonal antibodies. Proc Natl Acad Sci U S A. 1981; 78(8):5147-5150. (Immunogen).
4. Ripani E, Sacchetti A, Corda D, Alberti S. Human Trop-2 is a tumor-associated calcium signal transducer. Int J Cancer. 1998; 76(5):671-676. (Biology).
数据库链接
Entrez-Gene ID
4070
参考图片
Flow cytometric analysis of Trop-2. MCF7 cells (Human breast adenocarcinoma; ATCC HTB-22) were incubated with 0.25 µg of the Purified Mouse Anti-Human Trop-2 antibody (clone 162-46; shaded) or with a mouse IgG1 isotype control (Cat. No. 555746; unshaded) in BD Pharmingen™ Stain Buffer (Cat. No.554656) for 1 hr on ice. Cells were washed twice in Stain Buffer and incubated with 1 µg of a FITC Goat Anti-Mouse Ig secondary antibody (Cat. No.554001) in Stain Buffer for 1 hr on ice. Cells were washed twice and analyzed by flow cytometry.
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