BD Pharmingen- PE Mouse Anti-Human -2-Microglobulin_BD Pharmingen
产品信息
荧光素标记
PE
抗原名称
β2-Microglobulin
宿主
Mouse IgM, κ
简单描述
β2-microglobulin is the 12 kDa β chain which noncovalently associates with the 44 kDa α chain to form the HLA Class I antigen complex. This antibody reacts with free or complexed β2-microglobulin.
TÜ99 does not react with β2-microglobulin of other species. This antibody is suitable for staining formalin-fixed, paraffin-embedded tissue sections using TUF pretreatment.
商品描述
TÜ99
β2-microglobulin is the 12 kDa β chain which noncovalently associates with the 44 kDa α chain to form the HLA Class I antigen complex. This antibody reacts with free or complexed β2-microglobulin.
TÜ99 does not react with β2-microglobulin of other species. This antibody is suitable for staining formalin-fixed, paraffin-embedded tissue sections using TUF pretreatment.
同种型
Mouse IgM, κ
克隆号
克隆 TÜ99 (RUO)
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
20 µl
反应种属
Human (QC Testing)
目标/特异性
β2-Microglobulin
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
文献
文献
研发参考(3)
1. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
2. Desoye G, Dohr GA, Motter W, et al. Lack of HLA class I and class II antigens on human preimplantation embryos. J Immunol. 1988; 140(12):4157-4159. (Biology).
3. Dohr GA, Motter W, Leitinger S, et al. Lack of expression of HLA [corrected] class I and class II molecules on the human oocyte. J Immunol. 1987; 138(11):3766-3770. (Biology).
数据库链接
Entrez-Gene ID
567
参考图片
Flow cytometric analysis of β2-Microglobulin expression on peripheral blood lymphocytes. Whole blood was stained with either PE Mouse Anti-Human β2-Microglobulin (Cat. No. 551337; solid line histogram), or with PE Mouse IgM, κ Isotype Control (Cat. No.555584; dashed line histogram). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes.
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