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BD Pharmingen- FITC Mouse Anti-Human -2-Microglobulin_BD Pharmingen

艾美捷科技 • 2025年08月20日 10:13:31 • 抗体 • 阅读 45 • 标签
产品信息
荧光素标记
FITC
抗原名称
β2-Microglobulin
宿主
Mouse IgM, κ
简单描述
β2-microglobulin is the 12 kDa β chain which noncovalently associates with the 44 kDa α chain to form the HLA Class I antigen complex. This antibody reacts with free or complexed β2-microglobulin. TÜ99 does not react with β2-microglobulin of other species. This antibody is suitable for staining formalin-fixed, paraffin-embedded tissue sections using TUF pretreatment. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
商品描述
TÜ99 β2-microglobulin is the 12 kDa β chain which noncovalently associates with the 44 kDa α chain to form the HLA Class I antigen complex. This antibody reacts with free or complexed β2-microglobulin. TÜ99 does not react with β2-microglobulin of other species. This antibody is suitable for staining formalin-fixed, paraffin-embedded tissue sections using TUF pretreatment. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
同种型
Mouse IgM, κ
克隆号
克隆 TÜ99 (RUO)
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
20 µl
反应种属
Human (QC Testing)
目标/特异性
β2-Microglobulin
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(3) 1. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997. 2. Desoye G, Dohr GA, Motter W, et al. Lack of HLA class I and class II antigens on human preimplantation embryos. J Immunol. 1988; 140(12):4157-4159. (Biology). 3. Dohr GA, Motter W, Leitinger S, et al. Lack of expression of HLA [corrected] class I and class II molecules on the human oocyte. J Immunol. 1987; 138(11):3766-3770. (Biology).
数据库链接
Entrez-Gene ID
567

参考图片

Profile of peripheral blood lymphocytes analyzed by flow cytometry

Profile of peripheral blood lymphocytes analyzed by flow cytometry

Multiparameter flow cytometric analysis of β2-Microglobulin expression on human peripheral blood leucocyte populations. Human whole blood was stained with either FITC Mouse IgM, κ Isotype Control (Cat. No.555583; Left Plot) or FITC Mouse Anti-Human β2-Microglobulin (Cat. No. 551338; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The bivariate pseudocolor density plots showing correlated expression of β2-Microglobulin [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scattering characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.

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