BD Pharmingen- Purified Mouse Anti-Human CD77_BD Pharmingen
产品信息
抗原名称
CD77
宿主
Mouse BALB/c IgM, κ
免疫原
Initially Adherent Human Peripheral Blood Cells
简单描述
The 5B5 monoclonal antibody specifically binds to human CD77, a small neutral glycosphingolipid also referred to as globotriaosylceramide (Gb3), Burkitt 's lymphoma associated antigen (BLA), ceramide trihexoside (CTH), and Pk blood group antigen. CD77 is expressed on a subset of germinal center B-lymphocytes including centroblasts that are found with greater frequency in tonsil and mucosal lymphoid tissues and less frequently in peripheral blood, bone marrow and spleen. It has been described as a receptor for Shiga Toxin, produced by
Shigella dysenteriae
, and Verotoxin 1 (VT1), produced by some strains of
E. coli
. CD19 has been reported as a ligand for CD77. This interaction may function in adhesion and signal transduction at a specific stage of B cell differentiation. Studies have shown that cross-linking CD77 induces apoptosis in some B cell lines.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
商品描述
5B5
The 5B5 monoclonal antibody specifically binds to human CD77, a small neutral glycosphingolipid also referred to as globotriaosylceramide (Gb3), Burkitt 's lymphoma associated antigen (BLA), ceramide trihexoside (CTH), and Pk blood group antigen. CD77 is expressed on a subset of germinal center B-lymphocytes including centroblasts that are found with greater frequency in tonsil and mucosal lymphoid tissues and less frequently in peripheral blood, bone marrow and spleen. It has been described as a receptor for Shiga Toxin, produced by
Shigella dysenteriae
, and Verotoxin 1 (VT1), produced by some strains of
E. coli
. CD19 has been reported as a ligand for CD77. This interaction may function in adhesion and signal transduction at a specific stage of B cell differentiation. Studies have shown that cross-linking CD77 induces apoptosis in some B cell lines.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
同种型
Mouse BALB/c IgM, κ
克隆号
克隆 5B5 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Human (QC Testing)
目标/特异性
CD77
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(4)
1. Fyfe G, Cebra-Thomas JA, Mustain E, Davie JM, Alley CD, Nahm MH. Subpopulations of B lymphocytes in germinal centers. J Immunol. 1987; 139(7):2187-2194. (Biology).
2. Lagresle C, Bella C, Daniel PT, Krammer PH, Defrance T. Regulation of germinal center B cell differentiation. Role of the human APO-1/Fas (CD95) molecule. J Immunol. 1995; 154(11):5746-5756. (Biology).
3. Maloney MD, Lingwood CA. CD19 has a potential CD77 (globotriaosyl ceramide)-binding site with sequence similarity to verotoxin B-subunits: implications of molecular mimicry for B cell adhesion and enterohemorrhagic Escherichia coli pathogenesis. J Exp Med. 1994; 180(1):191-201. (Biology).
4. Mangeney M, Rousselet G, Taga S, Tursz T, Wiels J. The fate of human CD77+ germinal center B lymphocytes after rescue from apoptosis. Mol Immunol. 1995; 32(5):333-339. (Biology).
参考图片
Profile of Ramos cell line analyzed by flow cytometry. Second step staining with Cat. No. 555988.
Profile of Ramos cell line analyzed by flow cytometry. Second step staining with Cat. No. 555988.
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