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BD Pharmingen- Biotin Rat Anti-Mouse CD51_BD Pharmingen

艾美捷科技 • 2025年08月20日 10:29:48 • 抗体 • 阅读 7 • 标签
产品信息
抗原名称
CD51 (Integrin αV)
宿主
Rat SD, also known as Sprague-Dawley (outbred) IgG1, κ
免疫原
Mouse (BALB/c) IL-2-activated killer (LAK) Cells
简单描述
The RMV-7 monoclonal antibody specifically binds to CD51, the 140 kDa integrin αV chain. Heterodimers of CD51 with several integrin β chains function as receptors for extracellular matrix proteins. CD51/CD61 (αVβ3 integrin, vitronectin receptor) mediates adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, von Willebrand factor, and CD31 (PECAM-1). CD51 is expressed on activated T lymphocytes, polymorphonuclear granulocytes, blastocysts, and osteoclasts. CD51 is reportedly not detectable on mouse platelets using either H9.2B8 or RMV-7 antibody clones. CD51 also forms heterodimers with CD29 (integrin β1), integrins β5, β6, and β8 chains. αV integrins have diverse functions in development and homeostasis. The RMV-7 antibody reportedly blocks LAK-cell binding to vitronectin, fibronectin, fibrinogen, and CD31. Furthermore, the RMV-7 antibody reportedly inhibits LAK-cell cytoxicity against certain target cells by interfering with the binding of LAK cells to their target cells.
商品描述
RMV-7 The RMV-7 monoclonal antibody specifically binds to CD51, the 140 kDa integrin αV chain. Heterodimers of CD51 with several integrin β chains function as receptors for extracellular matrix proteins. CD51/CD61 (αVβ3 integrin, vitronectin receptor) mediates adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, von Willebrand factor, and CD31 (PECAM-1). CD51 is expressed on activated T lymphocytes, polymorphonuclear granulocytes, blastocysts, and osteoclasts. CD51 is reportedly not detectable on mouse platelets using either H9.2B8 or RMV-7 antibody clones. CD51 also forms heterodimers with CD29 (integrin β1), integrins β5, β6, and β8 chains. αV integrins have diverse functions in development and homeostasis. The RMV-7 antibody reportedly blocks LAK-cell binding to vitronectin, fibronectin, fibrinogen, and CD31. Furthermore, the RMV-7 antibody reportedly inhibits LAK-cell cytoxicity against certain target cells by interfering with the binding of LAK cells to their target cells.
同种型
Rat SD, also known as Sprague-Dawley (outbred) IgG1, κ
克隆号
克隆 RMV-7 (RUO)
浓度
0.5 mg/ml
产品详情
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
CD51 (Integrin αV)
背景
别名
Itgav; Integrin alpha-V; Integrin αV ; VNRA; Vitronectin receptor alpha
制备和贮存
存储溶液
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
文献
文献
研发参考(8) 1. Bader BL, Rayburn H, Crowley D, Hynes RO. Extensive vasculogenesis, angiogenesis, and organogenesis precede lethality in mice lacking all alpha v integrins. Cell. 1998; 95(4):507-519. (Biology). 2. Frieser M, Hallmann R, Johansson S, Vestweber D, Goodman SL, Sorokin L. Mouse polymorphonuclear granulocyte binding to extracellular matrix molecules involves beta 1 integrins. Eur J Immunol. 1996; 26(12):3127-3136. (Biology). 3. Moulder K, Roberts K, Shevach EM, Coligan JE. The mouse vitronectin receptor is a T cell activation antigen. J Exp Med. 1991; 173(2):343-347. (Biology). 4. Nakamura I, Pilkington MF, Lakkakorpi PT, et al. Role of alpha(v)beta(3) integrin in osteoclast migration and formation of the sealing zone. J Cell Sci. 1999; 112(22):3985-3993. (Biology). 5. Narumiya S, Abe Y, Kita Y, et al. Pre-B cells adhere to fibronectin via interactions of integrin alpha 5/alpha V with RGDS as well as of integrin alpha 4 with two distinct V region sequences at its different binding sites. Int Immunol. 1994; 6(1):139-147. (Biology: Blocking). 6. Piali L, Hammel P, Uherek C, et al. CD31/PECAM-1 is a ligand for alpha v beta 3 integrin involved in adhesion of leukocytes to endothelium. J Cell Biol. 1995; 130(2):451-460. (Biology: Blocking). 7. Schultz JF, Armant DR. Beta 1- and beta 3-class integrins mediate fibronectin binding activity at the surface of developing mouse peri-implantation blastocysts. Regulation by ligand-induced mobilization of stored receptor. J Biol Chem. 1995; 270(19):11522-11531. (Biology). 8. Takahashi K, Nakamura T, Koyanagi M, et al. A murine very late activation antigen-like extracellular matrix receptor involved in CD2- and lymphocyte function-associated antigen-1-independent killer-target cell interaction. J Immunol. 1990; 145(12):4371-4379. (Immunogen: Blocking, Immunoprecipitation).
数据库链接
Entrez-Gene ID
16410

参考图片

Flow cytometric analysis of CD51 on mouse bone marrow myeloid cells. BALB/c bone marrow leukocytes were either left unstained (left panel) or stained with Biotin Rat Anti-Mouse CD51 (Cat. No. 551380, right panel), followed by PE Streptavidin (Cat. No. 554061). Please note that the population of cells having the lowest SSC (erythroid and lymphoid cells) show little expression of CD51, while cells with moderate-to-high SSC (myeloid cells) are almost uniformly CD51 positive (right panel). The contour plots were based on the gated events with the forward and side light-scattering characteristics of viable leukocytes. Flow cytometry was performed on a BD FACScan™

Flow cytometric analysis of CD51 on mouse bone marrow myeloid cells. BALB/c bone marrow leukocytes were either left unstained (left panel) or stained with Biotin Rat Anti-Mouse CD51 (Cat. No. 551380, right panel), followed by PE Streptavidin (Cat. No. 554061). Please note that the population of cells having the lowest SSC (erythroid and lymphoid cells) show little expression of CD51, while cells with moderate-to-high SSC (myeloid cells) are almost uniformly CD51 positive (right panel). The contour plots were based on the gated events with the forward and side light-scattering characteristics of viable leukocytes. Flow cytometry was performed on a BD FACScan™

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