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BD Pharmingen- APC Rat Anti-Human IL-2_BD Pharmingen

艾美捷科技 • 2025年08月20日 10:30:42 • 抗体 • 阅读 21 • 标签
产品信息
荧光素标记
APC
抗原名称
IL-2
宿主
Rat IgG2a, κ
免疫原
Human IL-2 Recombinant Protein
简单描述
The MQ1-17H12 monoclonal antibody specifically binds to the multifunctional cytokine, human Interleukin-2 (IL-2). IL-2 is produced by activated T cells and has multiple functions that can affect the growth, proliferation, differentiation and survival of many different target cell types including T cells, B cells, NK cells, monocytes and macrophages. The immunogen used to generate the MQ1-17H12 hybridoma was purified recombinant human IL-2 protein. The MQ1-17H12 antibody reportedly neutralizes the biological activity of human IL-2.
商品描述
MQ1-17H12 The MQ1-17H12 monoclonal antibody specifically binds to the multifunctional cytokine, human Interleukin-2 (IL-2). IL-2 is produced by activated T cells and has multiple functions that can affect the growth, proliferation, differentiation and survival of many different target cell types including T cells, B cells, NK cells, monocytes and macrophages. The immunogen used to generate the MQ1-17H12 hybridoma was purified recombinant human IL-2 protein. The MQ1-17H12 antibody reportedly neutralizes the biological activity of human IL-2.
同种型
Rat IgG2a, κ
克隆号
克隆 MQ1-17H12 (RUO)
产品详情
APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
APC
Red 627-640 nm
651 nm
660 nm
应用
实验应用
Intracellular staining (flow cytometry) (Routinely Tested)
推荐用量
20 µl
反应种属
Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
目标/特异性
IL-2
背景
别名
IL2; Interleukin-2; T-cell growth factor; TCGF
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(4) 1. Meager A. Characterization of interferons and immunoassays. In: Clemens MJ, Morris AG, Gearing AJH, ed. Lymphokines and Interferons. A Practical Approach. Oxford: IRL Press Ltd; 1987:105-127. 2. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Clone-specific). 3. Sopper S, Stahl-Hennig C, Demuth M, Johnston IC, Dorries R, ter Meulen V. Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys. Cytometry. 1997; 29(4):351-362. (Biology). 4. Verdier F, Aujoulat M, Condevaux F, Descotes J. Determination of lymphocyte subsets and cytokine levels in cynomolgus monkeys. Toxicology. 1995; 105(1):81-90. (Biology).
数据库链接
Entrez-Gene ID
3558

参考图片

Profile of anti-IL-2 on PMA + CA++ ionophore-stimulated peripheral blood lymphocytes of Rhesus macaque monkey (macaca mulatta) analyzed by flow cytometry

Flow cytometric analysis of IL-2 expression on PMA+ CA++ ionophore-stimulated peripheral blood lymphocytes of Rhesus macaque (Macaca mulatta). PBMCs were stimulated for 6 hours with PMA (50 ng/ml final concentration; Sigma, Cat. #P-8139) and calcium ionophore A23187 (500-100 ng/ml final concentration; Sigma, Cat. #C-9275) in the presence of BD GolgiStop™ (2 μM final concentration; Cat. No. 554724). The PBMCs were stained with FITC Mouse Anti-Human CD3 (Cat. No. 555916), fixed, permeabilized, and then stained with APC Rat Anti-Human IL-2 (Cat. No. 551383). While the frequency of CD3+ non-human primate cells reacting with MQ1-17H12 following PMA + Ca++ ionophore activation is similar to that observed with normal human donor CD3+ lymphocytes, the fluorescence intensity is characteristically weaker.

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