1. 首页
  2. 抗体
  3. 正文

BD Pharmingen- APC Mouse Anti-Human TNF_BD Pharmingen

艾美捷科技 • 2025年08月20日 10:31:33 • 抗体 • 阅读 73 • 标签
产品信息
荧光素标记
APC
抗原名称
TNF
宿主
Mouse IgG1, κ
免疫原
Recombinant Human TNF
简单描述
Clone MAb11 reacts with the human form of tumor necrosis factor (TNF, formerly known as TNF-α) detectable in the cytoplasm of a major subset of activated peripheral blood T lymphocytes. Using standard procedures for detection of intracellular proteins, clone MAb11 also cross-reacts with activated peripheral blood CD3+ lymphocytes of baboon, and both rhesus and cynomolgus macaque monkeys following five-hour treatment with phorbol myristic acetate (PMA) and Ca++ Ionophore (A23187) in the presence of monensin. The staining is restricted to CD3+ T cells and is similar to that observed with peripheral blood T lymphocytes from normal human donors.
商品描述
MAb11 Clone MAb11 reacts with the human form of tumor necrosis factor (TNF, formerly known as TNF-α) detectable in the cytoplasm of a major subset of activated peripheral blood T lymphocytes. Using standard procedures for detection of intracellular proteins, clone MAb11 also cross-reacts with activated peripheral blood CD3+ lymphocytes of baboon, and both rhesus and cynomolgus macaque monkeys following five-hour treatment with phorbol myristic acetate (PMA) and Ca++ Ionophore (A23187) in the presence of monensin. The staining is restricted to CD3+ T cells and is similar to that observed with peripheral blood T lymphocytes from normal human donors.
同种型
Mouse IgG1, κ
克隆号
克隆 MAb11 (RUO)
产品详情
APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
APC
Red 627-640 nm
651 nm
660 nm
应用
实验应用
Intracellular staining (flow cytometry) (Routinely Tested)
推荐用量
20 µl
反应种属
Human, Rhesus, Cynomolgus, Baboon (QC Testing)
目标/特异性
TNF
背景
别名
Tumor necrosis factor alpha; TNF-a; TNF-α; TNFSF2; Cachectin
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(6) 1. Danis VA, Franic GM, Rathjen DA, Brooks PM. Effects of granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-2, interferon-gamma (IFN-gamma), tumour necrosis factor-alpha (TNF-alpha) and IL-6 on the production of immunoreactive IL-1 and TNF-alpha by human monocytes. Clin Exp Immunol. 1991; 85(1):143-150. (Clone-specific: ELISA). 2. Meager A. Characterization of interferons and immunoassays. In: Clemens MJ, Morris AG, Gearing AJH, ed. Lymphokines and Interferons. A Practical Approach. Oxford: IRL Press Ltd; 1987:105-127. 3. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology). 4. Rathjen DA, Cowan K, Furphy LJ, Aston R. Antigenic structure of human tumour necrosis factor: recognition of distinct regions of TNF alpha by different tumour cell receptors. Mol Immunol. 1991; 28(1-2):79-86. (Clone-specific: ELISA). 5. Sopper S, Stahl-Hennig C, Demuth M, Johnston IC, Dorries R, ter Meulen V. Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys. Cytometry. 1997; 29(4):351-362. (Biology). 6. Verdier F, Aujoulat M, Condevaux F, Descotes J. Determination of lymphocyte subsets and cytokine levels in cynomolgus monkeys. Toxicology. 1995; 105(1):81-90. (Biology).

参考图片

Flow cytometric profile of TNF expression on rhesus macaque (Macaca mulatta) peripheral blood lymphocytes. Peripheral blood lymphocytes were stimulated with PMA, then fixed, permeabilized, and washed with the BD Cytofix/Cytoperm Plus Kit (with BD GolgiStop)(Cat. No. 554715). Samples were then costained with APC Mouse Anti-Human TNF (Cat. No. 551384) and FITC Mouse Anti-Human CD3ε (Cat. No. 556611). The two-color dot plot was derived from gated events with the forward and side-light scatter characteristics of viable lymphocytes.

Flow cytometric profile of TNF expression on rhesus macaque (Macaca mulatta) peripheral blood lymphocytes. Peripheral blood lymphocytes were stimulated with PMA, then fixed, permeabilized, and washed with the BD Cytofix/Cytoperm Plus Kit (with BD GolgiStop)(Cat. No. 554715). Samples were then costained with APC Mouse Anti-Human TNF (Cat. No. 551384) and  FITC Mouse Anti-Human CD3ε (Cat. No. 556611). The two-color dot plot was derived from gated events with the forward and side-light scatter characteristics of viable lymphocytes.

文档下载: W 导出为BD Pharmingen- APC Mouse Anti-Human TNF_BD Pharmingen.doc文档

本文来自投稿，不代表本人立场，如若转载，请注明出处：http://www.iamyjet.com/kangti/8946.html

赞 (73)
打赏 微信扫一扫
« 上一篇 2025年08月20日 10:30:42
下一篇 » 2025年08月20日 10:32:09

相关推荐

×
(function(){ var src = (document.location.protocol == "http:") ? "http://js.passport.qihucdn.com/11.0.1.js?1d7dde81dc0903e04d3ac0b9599444f6":"https://jspassport.ssl.qhimg.com/11.0.1.js?1d7dde81dc0903e04d3ac0b9599444f6"; document.write('<\/mip-script>'); })(); (function(){ var bp = document.createElement('script'); var curProtocol = window.location.protocol.split(':')[0]; if (curProtocol === 'https') { bp.src = 'https://zz.bdstatic.com/linksubmit/push.js'; } else { bp.src = 'http://push.zhanzhang.baidu.com/push.js'; } var s = document.getElementsByTagName("script")[0]; s.parentNode.insertBefore(bp, s); })();