BD Pharmingen- PerCP-Cy-5-5 Streptavidin_BD Pharmingen
产品信息
荧光素标记
PerCP-Cy5.5
抗原名称
Streptavidin
简单描述
PerCP-Cy5.5 is a tandem fluorochrome composed of peridinin chlorophyll protein (PerCP), which is excited by the 488-nm line of an Argon ion laser and serves as the energy donor, coupled to the cyanine dye Cy™5.5, which acts as the energy acceptor and fluoresces at 695 nm.
SAv-PerCP-Cy5.5 is a useful second-step reagent for the indirect immunofluorescent staining of cells in combination with biotinylated primary antibodies for flow cytometric analysis. PerCP-Cy5.5 tandem fluorochrome emission is collected in the Fluorescence-3 (FL3) channel of BD FACScan™ and BD FACSCalibur™ flow cytometry systems.
商品描述
PerCP-Cy5.5 is a tandem fluorochrome composed of peridinin chlorophyll protein (PerCP), which is excited by the 488-nm line of an Argon ion laser and serves as the energy donor, coupled to the cyanine dye Cy™5.5, which acts as the energy acceptor and fluoresces at 695 nm.
SAv-PerCP-Cy5.5 is a useful second-step reagent for the indirect immunofluorescent staining of cells in combination with biotinylated primary antibodies for flow cytometric analysis. PerCP-Cy5.5 tandem fluorochrome emission is collected in the Fluorescence-3 (FL3) channel of BD FACScan™ and BD FACSCalibur™ flow cytometry systems.
克隆号
(RUO)
浓度
0.2 mg/ml
产品详情
PerCP-Cy5.5
PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PerCP-Cy5.5
Blue 488 nm
482 nm
676 nm
应用
实验应用
Flow cytometry (Routinely Tested)
目标/特异性
Streptavidin
制备和贮存
存储溶液
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
文献
文献
研发参考(2)
1. Greimers R, Trebak M, Moutschen M, Jacobs N, Boniver J. Improved four-color flow cytometry method using fluo-3 and triple immunofluorescence for analysis of intracellular calcium ion ([Ca2+]i) fluxes among mouse lymph node B- and T-lymphocyte subsets. Cytometry. 1996; 23(3):205-217. (Biology).
2. Shapiro HM. Practical Flow Cytometry, 3rd Edition. New York: Wiley-Liss, Inc; 1995:280-281.
参考图片
PerCP-Cy5.5 spectra. The absorption spectrum of Streptavidin-PerCP-Cy5.5 is presented in the left panel. The corresponding emission spectrum, at the excitation wavelength of 488 nm, appears in the right panel.
PerCP-Cy5.5 spectra. The absorption spectrum of Streptavidin-PerCP-Cy5.5 is presented in the left panel. The corresponding emission spectrum, at the excitation wavelength of 488 nm, appears in the right panel.
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